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dc.contributor.authorSaid, Mahmoud
dc.contributor.authorHolušová, Katerina
dc.contributor.authorFarkas, András
dc.contributor.authorIvanizs, László
dc.contributor.authorGaál, Eszter
dc.contributor.authorCápal, Petr
dc.contributor.authorAbrouk, Michael
dc.contributor.authorMartis-Thiele, Mihaela M
dc.contributor.authorKalapos, Balázs
dc.contributor.authorBartoš, Jan
dc.contributor.authorFriebe, Bernd
dc.contributor.authorDoležel, Jaroslav
dc.contributor.authorMolnár, István
dc.date.accessioned2021-07-14T11:12:48Z
dc.date.available2021-07-14T11:12:48Z
dc.date.issued2021-06-15
dc.date.submitted2021-03-31
dc.identifier.citationSaid, M., Holušová, K., Farkas, A., Ivanizs, L., Gaál, E., Cápal, P., … Molnár, I. (2021). Development of DNA Markers From Physically Mapped Loci in Aegilops comosa and Aegilops umbellulata Using Single-Gene FISH and Chromosome Sequences. Frontiers in Plant Science, 12. doi:10.3389/fpls.2021.689031
dc.identifier.issn1664-462X
dc.identifier.issn1664-462X
dc.identifier.issn1664-462X
dc.identifier.pmid34211490
dc.identifier.doi10.3389/fpls.2021.689031
dc.identifier.urihttp://hdl.handle.net/10754/670214
dc.description.abstractBreeding of agricultural crops adapted to climate change and resistant to diseases and pests is hindered by a limited gene pool because of domestication and thousands of years of human selection. One way to increase genetic variation is chromosome-mediated gene transfer from wild relatives by cross hybridization. In the case of wheat (Triticum aestivum), the species of genus Aegilops are a particularly attractive source of new genes and alleles. However, during the evolution of the Aegilops and Triticum genera, diversification of the D-genome lineage resulted in the formation of diploid C, M, and U genomes of Aegilops. The extent of structural genome alterations, which accompanied their evolution and speciation, and the shortage of molecular tools to detect Aegilops chromatin hamper gene transfer into wheat. To investigate the chromosome structure and help develop molecular markers with a known physical position that could improve the efficiency of the selection of desired introgressions, we developed single-gene fluorescence in situ hybridization (FISH) maps for M- and U-genome progenitors, Aegilops comosa and Aegilops umbellulata, respectively. Forty-three ortholog genes were located on 47 loci in Ae. comosa and on 52 loci in Ae. umbellulata using wheat cDNA probes. The results obtained showed that M-genome chromosomes preserved collinearity with those of wheat, excluding 2 and 6M containing an intrachromosomal rearrangement and paracentric inversion of 6ML, respectively. While Ae. umbellulata chromosomes 1, 3, and 5U maintained collinearity with wheat, structural reorganizations in 2, 4, 6, and 7U suggested a similarity with the C genome of Aegilops markgrafii. To develop molecular markers with exact physical positions on chromosomes of Aegilops, the single-gene FISH data were validated in silico using DNA sequence assemblies from flow-sorted M- and U-genome chromosomes. The sequence similarity search of cDNA sequences confirmed 44 out of the 47 single-gene loci in Ae. comosa and 40 of the 52 map positions in Ae. umbellulata. Polymorphic regions, thus, identified enabled the development of molecular markers, which were PCR validated using wheat-Aegilops disomic chromosome addition lines. The single-gene FISH-based approach allowed the development of PCR markers specific for cytogenetically mapped positions on Aegilops chromosomes, substituting as yet unavailable segregating map. The new knowledge and resources will support the efforts for the introgression of Aegilops genes into wheat and their cloning.
dc.description.sponsorshipThe authors would like to thank Dr. Shuhei Nasuda from the Graduate School of Agriculture, Kyoto University, Japan, and Dr. Tatiana Danilova from Kansas State University, USA, for providing the cDNA clones. Z. Dubská, R. Šperková, and J. Weiserová are acknowledged for their assistance with chromosome sorting and preparation of DNA for sequencing.
dc.publisherFrontiers Media SA
dc.relation.urlhttps://www.frontiersin.org/articles/10.3389/fpls.2021.689031/full
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectMolecular markers
dc.subjectAegilops umbellulata
dc.subjectChromosome Rearrangements
dc.subjectAegilops Comosa
dc.subjectChromosome Flow Sorting And Sequencing
dc.subjectGoat Grasses
dc.subjectHomoeologous Relationships
dc.subjectSingle-Gene Fish
dc.titleDevelopment of DNA Markers From Physically Mapped Loci in Aegilops comosa and Aegilops umbellulata Using Single-Gene FISH and Chromosome Sequences
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalFrontiers in plant science
dc.identifier.pmcidPMC8240756
dc.eprint.versionPublisher's Version/PDF
dc.identifier.volume12
kaust.personAbrouk, Michael
dc.date.accepted2021-03-19
refterms.dateFOA2021-07-14T11:13:41Z


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This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original
publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.