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dc.contributor.authorLi, Mo
dc.contributor.authorZhao, Qing
dc.contributor.authorBelloli, Ryan
dc.contributor.authorDuffy, Carly R.
dc.contributor.authorCai, Haini N.
dc.date.accessioned2021-07-14T07:21:19Z
dc.date.available2021-07-14T07:21:19Z
dc.date.issued2021-04-20
dc.date.submitted2020-09-23
dc.identifier.citationLi, M., Zhao, Q., Belloli, R., Duffy, C. R., & Cai, H. N. (2021). Insulator foci distance correlates with cellular and nuclear morphology in early Drosophila embryos. Developmental Biology, 476, 189–199. doi:10.1016/j.ydbio.2021.03.022
dc.identifier.issn1095-564X
dc.identifier.issn0012-1606
dc.identifier.doi10.1016/j.ydbio.2021.03.022
dc.identifier.urihttp://hdl.handle.net/10754/670207
dc.description.abstractThe three-dimensional (3D) organization of the genome is highly dynamic, changing during development and varying across different tissues and cell types. Recent studies indicate that these changes alter regulatory interactions, leading to changes in gene expression. Despite its importance, the mechanisms that influence genomic organization remain poorly understood. We have previously identified a network of chromatin boundary elements, or insulators, in the Drosophila Antennapedia homeotic complex (ANT-C). These genomic elements interact with one another to tether chromatin loops that could block or promote enhancer-promoter interactions. To understand the function of these insulators, we assessed their interactions by measuring their 3D nuclear distance in developing animal tissues. Our data suggest that the ANT-C Hox complex might be in a folded or looped configuration rather than in a random or extended form. The architecture of the ANT-C complex, as read out by the pair-wise distance between insulators, undergoes a strong compression during late embryogenesis, coinciding with the reduction of cell and nuclear diameters due to continued cell divisions in post-cleavage cells. Our results suggest that genomic architecture and gene regulation may be influenced by cellular morphology and movement during development.
dc.publisherElsevier BV
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S0012160621000841
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in DEVELOPMENTAL BIOLOGY. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in DEVELOPMENTAL BIOLOGY, [476, , (2021-04-20)] DOI: 10.1016/j.ydbio.2021.03.022 . © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectNuclear fluorescent in situ hybridization
dc.subjectHomeotic genes
dc.subjectAntennapedia complex
dc.subjectChromatin boundary element
dc.subjectInsulator
dc.subjectSF1
dc.subjectAU1
dc.subjectChromatin loop
dc.subjectDrosophila
dc.subjectCell and nuclear morphology
dc.titleInsulator foci distance correlates with cellular and nuclear morphology in early Drosophila embryos
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.identifier.journalDEVELOPMENTAL BIOLOGY
dc.rights.embargodate2023-04-20
dc.identifier.wosutWOS:000656764100005
dc.eprint.versionPost-print
dc.contributor.institutionDepartment of Cellular Biology, University of Georgia, Athens GA, 30602, USA
dc.identifier.volume476
dc.identifier.pages189-199
kaust.personLi, Mo
dc.date.accepted2021-03-26
dc.identifier.eid2-s2.0-85104445493
dc.date.published-online2021-04-20
dc.date.published-print2021-08


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