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dc.contributor.authorAlves, Ricardo N.
dc.contributor.authorRambla-Alegre, Maria
dc.contributor.authorBraga, Ana Catarina
dc.contributor.authorMauhrault, Ana L.
dc.contributor.authorBarbosa, Vera
dc.contributor.authorCampas, Monica
dc.contributor.authorReverte, Laia
dc.contributor.authorFlores, Cintia
dc.contributor.authorCaixach, Josep
dc.contributor.authorKilcoyne, Jane
dc.contributor.authorCosta, Pedro Reis
dc.contributor.authorDiogene, Jorge
dc.contributor.authorMarque, Antonio
dc.date.accessioned2021-07-11T10:41:20Z
dc.date.available2021-07-11T10:41:20Z
dc.date.issued2019
dc.identifier.citationAlves, R. N., Rambla-Alegre, M., Braga, A. C., Maulvault, A. L., Barbosa, V., Campàs, M., … Marques, A. (2019). Bioaccessibility of lipophilic and hydrophilic marine biotoxins in seafood: An in vitro digestion approach. Food and Chemical Toxicology, 129, 153–161. doi:10.1016/j.fct.2019.04.041
dc.identifier.issn1873-6351
dc.identifier.issn0278-6915
dc.identifier.doi10.1016/j.fct.2019.04.041
dc.identifier.urihttp://hdl.handle.net/10754/670108
dc.description.abstractThis study aimed to assess the bioaccessibility of different marine biotoxins in naturally contaminated shellfish and fish gonads using an in vitro digestion methodology. In general, hydrophilic toxins (domoic acid, paralytic shellfish poisoning toxins and tetrodotoxins) showed higher bioaccessibility than lipophilic ones (okadaic acid and azaspiracids). The bioaccessibility of toxins from the okadaic acid group ranged from 69% (raw European razor clams) to 74% (raw donax clams). Regarding azaspiracids, 47% of the initial content was bioaccessible in steamed blue mussel. As for hydrophilic toxins, 100% of the initial content was bioaccessible after digestion in raw shellfish and puffer fish gonads. The total tetrodotoxin bioaccessibility in puffer fish gonads decreased significantly after steaming. The profile of tetrodotoxins changed during the digestion process: TTX and 11-norTTX-6S-ol analogues decreased significantly after digestion, but the 5,6,11-trideoxy TTX analogue increased in both raw and steamed puffer fish gonads. These preliminary findings confirm the need to consider bioaccessibility data in future seafood risk assessment, as such information enables a more accurate and realistic estimation of potential seafood hazards, particularly in what concerns lipophilic toxins, therefore, constituting a crucial tool in the refinement of regulatory limits for the presence of biotoxins in seafood.
dc.description.sponsorshipThe research leading to these results has received funding from the European Union Seventh Framework Programme (FP7/2007–2013) under the ECsafeSEAFOOD project (grant agreement n° 311820) and through the European Regional Development Fund. IRTA members acknowledge support from CERCA Programme/Generalitat de Catalunya. L.R. acknowledges a scholarship from the ECsafeSEAFOOD project. Ana C. Braga has a Doctoral Grant (PD/BD/113484/2015) from the Portuguese Science and Technology Foundation (FCT), whereas Antonio Marques and Pedro R. Costa are supported through the FCT Investigator program (IF). Lagocephalus sceleratus was kindly provided by Andrés Izquierdo-Muñoz from Centro de Investigación Marina de Santa Pola (CIMA) (Spain).
dc.publisherElsevier BV
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S0278691519302406
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in FOOD AND CHEMICAL TOXICOLOGY. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in FOOD AND CHEMICAL TOXICOLOGY, [129, , (2019)] DOI: 10.1016/j.fct.2019.04.041 . © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectSeafood
dc.subjectBioaccessibility
dc.subjectOkadaic acid
dc.subjectAzaspiracids
dc.subjectDomoic acid
dc.subjectPSP toxins
dc.subjectTetrodotoxins
dc.titleBioaccessibility of lipophilic and hydrophilic marine biotoxins in seafood: An in vitro digestion approach
dc.typeArticle
dc.identifier.journalFOOD AND CHEMICAL TOXICOLOGY
dc.rights.embargodate2021
dc.identifier.wosutWOS:000472686200016
dc.eprint.versionPost-print
dc.contributor.institutionIPMA - Portuguese Institute of the Sea and Atmosphere, Av. Brasília, 1449-006, Lisbon, Portugal
dc.contributor.institutionIRTA, Ctra. Poble Nou, Km. 5.5, 43540, Sant Carles de La Ràpita, Spain
dc.contributor.institutionDepartment of Biology & CESAM – Centre for Environmental and Marine Studies, University of Aveiro, 3810-193, Aveiro, Portugal
dc.contributor.institutionInterdisciplinary Centre of Marine and Environmental Research (CIIMAR), University of Porto, Porto, Portugal
dc.contributor.institutionMARE – Marine and Environmental Sciences Centre, Faculty of Sciences, University of Lisbon (FCUL), Lisboa, Portugal
dc.contributor.institutionMass Spectrometry Laboratory/Organic Pollutants, IDAEA-CSIC, Jordi Girona 18, 08034, Barcelona, Spain
dc.contributor.institutionMarine Institute, Rinville, Oranmore, County Galway, Ireland
dc.contributor.institutionCCMAR – Centre of Marine Sciences, University of Algarve, Campus of Gambelas, 8005-139, Faro, Portugal
dc.identifier.volume129
dc.identifier.pages153-161
dc.identifier.eid2-s2.0-85064932440


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