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dc.contributor.authorZeeshan, Mohammad
dc.contributor.authorPandey, Rajan
dc.contributor.authorSubudhi, Amit
dc.contributor.authorFerguson, David J P
dc.contributor.authorKaur, Gursimran
dc.contributor.authorRashpa, Ravish
dc.contributor.authorNugmanova, Raushan
dc.contributor.authorBrady, Declan
dc.contributor.authorBottrill, Andrew R.
dc.contributor.authorVaughan, Sue
dc.contributor.authorBrochet, Mathieu
dc.contributor.authorBollen, Mathieu
dc.contributor.authorPain, Arnab
dc.contributor.authorHolder, Anthony A.
dc.contributor.authorGuttery, David S.
dc.contributor.authorTewari, Rita
dc.date.accessioned2021-06-20T13:36:40Z
dc.date.available2021-06-20T13:36:40Z
dc.date.issued2021-06-18
dc.date.submitted2021-01-18
dc.identifier.citationZeeshan, M., Pandey, R., Subudhi, A. K., Ferguson, D. J. P., Kaur, G., Rashpa, R., … Tewari, R. (2021). Protein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages. Communications Biology, 4(1). doi:10.1038/s42003-021-02273-0
dc.identifier.issn2399-3642
dc.identifier.doi10.1038/s42003-021-02273-0
dc.identifier.urihttp://hdl.handle.net/10754/669707
dc.description.abstractAbstractPP1 is a conserved eukaryotic serine/threonine phosphatase that regulates many aspects of mitosis and meiosis, often working in concert with other phosphatases, such as CDC14 and CDC25. The proliferative stages of the malaria parasite life cycle include sexual development within the mosquito vector, with male gamete formation characterized by an atypical rapid mitosis, consisting of three rounds of DNA synthesis, successive spindle formation with clustered kinetochores, and a meiotic stage during zygote to ookinete development following fertilization. It is unclear how PP1 is involved in these unusual processes. Using real-time live-cell and ultrastructural imaging, conditional gene knockdown, RNA-seq and proteomic approaches, we show that Plasmodium PP1 is implicated in both mitotic exit and, potentially, establishing cell polarity during zygote development in the mosquito midgut, suggesting that small molecule inhibitors of PP1 should be explored for blocking parasite transmission.
dc.description.sponsorshipWe thank Julie Rodgers for helping to maintain the insectary and other technical works, and the personnel at the Bioscience Core Laboratory (BCL) in KAUST for sequencing the RNA samples and producing the raw datasets. This work was supported by: MRC UK (G0900278, MR/K011782/1, MR/N023048/1) and BBSRC (BB/N017609/1) to R.T. and M.Z.; the Francis Crick Institute (FC001097), the Cancer Research UK (FC001097), the UK Medical Research Council (FC001097), and the Wellcome Trust (FC001097) to A.A.H.; the Swiss National Science Foundation project grant 31003A_179321 to M.Br; a faculty baseline fund (BAS/1/1020-01-01) and a Competitive Research Grant (CRG) award from OSR (OSR-2018-CRG6-3392) from the King Abdullah University of Science and Technology to A.P.; M.Br is an INSERM and EMBO young investigator. This research was funded in whole, or in part, by the Wellcome Trust [FC001097]. For the purpose of Open Access, the author has applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission.
dc.publisherSpringer Science and Business Media LLC
dc.relation.urlhttp://www.nature.com/articles/s42003-021-02273-0
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleProtein phosphatase 1 regulates atypical mitotic and meiotic division in Plasmodium sexual stages
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentBioscience
dc.contributor.departmentBioscience Program
dc.contributor.departmentPathogen Genomics Laboratory
dc.identifier.journalCommunications Biology
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionSchool of Life Sciences, University of Nottingham, Nottingham, UK.
dc.contributor.institutionNuffield Department of Clinical Laboratory Sciences, University of Oxford, John Radcliffe Hospital, Oxford, UK.
dc.contributor.institutionDepartment of Biological and Medical Sciences, Faculty of Health and Life Science, Oxford Brookes University, Gipsy Lane, Oxford, UK.
dc.contributor.institutionDepartment of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
dc.contributor.institutionSchool of Life Sciences, Gibbet Hill Campus, University of Warwick, Coventry, UK.
dc.contributor.institutionLaboratory of Biosignaling and Therapeutics, KU Leuven Department of Cellular and Molecular Medicine, University of Leuven, Leuven, Belgium.
dc.contributor.institutionResearch Center for Zoonosis Control, Global Institution for Collaborative Research and Education (GI-CoRE); Hokkaido University, Sapporo, Japan.
dc.contributor.institutionMalaria Parasitology Laboratory, The Francis Crick Institute, London, UK.
dc.contributor.institutionLeicester Cancer Research Centre, University of Leicester, Leicester, UK.
dc.identifier.volume4
dc.identifier.issue1
kaust.personSubudhi, Amit
kaust.personNugmanova, Raushan
kaust.personPain, Arnab
kaust.grant.numberBAS/1/1020-01-01
kaust.grant.numberCRG
kaust.grant.numberOSR-2018-CRG6-3392
dc.date.accepted2021-05-25
refterms.dateFOA2021-06-20T13:38:15Z
kaust.acknowledged.supportUnitBAS
kaust.acknowledged.supportUnitBioscience Core Laboratory (BCL)
kaust.acknowledged.supportUnitCompetitive Research Grant
kaust.acknowledged.supportUnitCRG
kaust.acknowledged.supportUnitOSR
dc.date.published-online2021-06-18
dc.date.published-print2021-12


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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
Except where otherwise noted, this item's license is described as This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.