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dc.contributor.authorAbd El Ghany, Moataz
dc.contributor.authorBarquist, Lars
dc.contributor.authorClare, Simon
dc.contributor.authorBrandt, Cordelia
dc.contributor.authorMayho, Matthew
dc.contributor.authorJoffre´, Enrique
dc.contributor.authorSjöling, Åsa
dc.contributor.authorTurner, A. Keith
dc.contributor.authorKlena, John D.
dc.contributor.authorKingsley, Robert A.
dc.contributor.authorHill-Cawthorne, Grant A.
dc.contributor.authorDougan, Gordon
dc.contributor.authorPickard, Derek
dc.date.accessioned2021-06-15T13:54:35Z
dc.date.available2021-06-15T13:54:35Z
dc.date.issued2021-06-10
dc.date.submitted2020-12-14
dc.identifier.citationAbd El Ghany, M., Barquist, L., Clare, S., Brandt, C., Mayho, M., Joffre´, E., … Pickard, D. (2021). Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization. Microbial Genomics, 7(6). doi:10.1099/mgen.0.000554
dc.identifier.issn2057-5858
dc.identifier.pmid34110281
dc.identifier.doi10.1099/mgen.0.000554
dc.identifier.urihttp://hdl.handle.net/10754/669590
dc.description.abstractEnterotoxigenic Escherichia coli (ETEC) expressing the colonization pili CFA/I are common causes of diarrhoeal infections in humans. Here, we use a combination of transposon mutagenesis and transcriptomic analysis to identify genes and pathways that contribute to ETEC persistence in water environments and colonization of a mammalian host. ETEC persisting in water exhibit a distinct RNA expression profile from those growing in richer media. Multiple pathways were identified that contribute to water survival, including lipopolysaccharide biosynthesis and stress response regulons. The analysis also indicated that ETEC growing in vivo in mice encounter a bottleneck driving down the diversity of colonizing ETEC populations.
dc.description.sponsorshipThe authors would like to thank KAUST Bioscience Core Laboratory for the support with generating sequencing data. The authors wish to thank the WTSI Sequencing team for their help with generating TraDIS sequencing data and the Pathogen Informatics Team for their assistance with bioinformatics analysis of TraDIS and RNAseq data.
dc.publisherMicrobiology Society
dc.relation.urlhttps://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000554
dc.rightsThis is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/legalcode
dc.titleFunctional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentCommunication Theory Lab
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer, Electrical and Mathematical Science and Engineering (CEMSE) Division
dc.contributor.departmentExtreme Systems Microbiology Lab
dc.contributor.departmentReef Ecology Lab
dc.identifier.journalMicrobial Genomics
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionMarie Bashir Institute for Infectious Diseases and Biosecurity, University of Sydney, Sydney, Australia
dc.contributor.institutionThe Westmead Institute for Medical Research, University of Sydney, Sydney, Australia
dc.contributor.institutionThe Westmead Clinical School, Faculty of Medicine and Health, University of Sydney, Sydney, Australia
dc.contributor.institutionFaculty of Medicine, University of Würzburg, Würzburg, Germany
dc.contributor.institutionHelmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), Würzburg, Germany
dc.contributor.institutionThe Wellcome Trust Sanger Institute (WTSI), the Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
dc.contributor.institutionDepartment of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden
dc.contributor.institutionQuadram Institute Bioscience, Norwich Research Park, Norwich, UK
dc.contributor.institutionCenters for Disease Control and Prevention, Atlanta, Georgia, USA
dc.contributor.institutionSchool of Public Health, the University of Sydney, Sydney, Australia
dc.contributor.institutionDepartment of Medicine, University of Cambridge, Cambridge, UK
dc.identifier.volume7
dc.identifier.issue6
kaust.personAbd El Ghany, Moataz
dc.date.accepted2021-03-09
refterms.dateFOA2021-06-15T13:55:29Z
kaust.acknowledged.supportUnitKAUST Bioscience Core Laboratory


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This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.