Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization
dc.contributor.author | Abd El Ghany, Moataz | |
dc.contributor.author | Barquist, Lars | |
dc.contributor.author | Clare, Simon | |
dc.contributor.author | Brandt, Cordelia | |
dc.contributor.author | Mayho, Matthew | |
dc.contributor.author | Joffre´, Enrique | |
dc.contributor.author | Sjöling, Åsa | |
dc.contributor.author | Turner, A. Keith | |
dc.contributor.author | Klena, John D. | |
dc.contributor.author | Kingsley, Robert A. | |
dc.contributor.author | Hill-Cawthorne, Grant A. | |
dc.contributor.author | Dougan, Gordon | |
dc.contributor.author | Pickard, Derek | |
dc.date.accessioned | 2021-06-15T13:54:35Z | |
dc.date.available | 2021-06-15T13:54:35Z | |
dc.date.issued | 2021-06-10 | |
dc.date.submitted | 2020-12-14 | |
dc.identifier.citation | Abd El Ghany, M., Barquist, L., Clare, S., Brandt, C., Mayho, M., Joffre´, E., … Pickard, D. (2021). Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization. Microbial Genomics, 7(6). doi:10.1099/mgen.0.000554 | |
dc.identifier.issn | 2057-5858 | |
dc.identifier.pmid | 34110281 | |
dc.identifier.doi | 10.1099/mgen.0.000554 | |
dc.identifier.uri | http://hdl.handle.net/10754/669590 | |
dc.description.abstract | Enterotoxigenic Escherichia coli (ETEC) expressing the colonization pili CFA/I are common causes of diarrhoeal infections in humans. Here, we use a combination of transposon mutagenesis and transcriptomic analysis to identify genes and pathways that contribute to ETEC persistence in water environments and colonization of a mammalian host. ETEC persisting in water exhibit a distinct RNA expression profile from those growing in richer media. Multiple pathways were identified that contribute to water survival, including lipopolysaccharide biosynthesis and stress response regulons. The analysis also indicated that ETEC growing in vivo in mice encounter a bottleneck driving down the diversity of colonizing ETEC populations. | |
dc.description.sponsorship | The authors would like to thank KAUST Bioscience Core Laboratory for the support with generating sequencing data. The authors wish to thank the WTSI Sequencing team for their help with generating TraDIS sequencing data and the Pathogen Informatics Team for their assistance with bioinformatics analysis of TraDIS and RNAseq data. | |
dc.publisher | Microbiology Society | |
dc.relation.url | https://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000554 | |
dc.rights | This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution. | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc/4.0/legalcode | |
dc.title | Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization | |
dc.type | Article | |
dc.contributor.department | Biological and Environmental Science and Engineering (BESE) Division | |
dc.contributor.department | Communication Theory Lab | |
dc.contributor.department | Computational Bioscience Research Center (CBRC) | |
dc.contributor.department | Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division | |
dc.contributor.department | Extreme Systems Microbiology Lab | |
dc.contributor.department | Reef Ecology Lab | |
dc.identifier.journal | Microbial Genomics | |
dc.eprint.version | Publisher's Version/PDF | |
dc.contributor.institution | Marie Bashir Institute for Infectious Diseases and Biosecurity, University of Sydney, Sydney, Australia | |
dc.contributor.institution | The Westmead Institute for Medical Research, University of Sydney, Sydney, Australia | |
dc.contributor.institution | The Westmead Clinical School, Faculty of Medicine and Health, University of Sydney, Sydney, Australia | |
dc.contributor.institution | Faculty of Medicine, University of Würzburg, Würzburg, Germany | |
dc.contributor.institution | Helmholtz Institute for RNA-based Infection Research (HIRI), Helmholtz Centre for Infection Research (HZI), Würzburg, Germany | |
dc.contributor.institution | The Wellcome Trust Sanger Institute (WTSI), the Wellcome Trust Genome Campus, Hinxton, Cambridge, UK | |
dc.contributor.institution | Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden | |
dc.contributor.institution | Quadram Institute Bioscience, Norwich Research Park, Norwich, UK | |
dc.contributor.institution | Centers for Disease Control and Prevention, Atlanta, Georgia, USA | |
dc.contributor.institution | School of Public Health, the University of Sydney, Sydney, Australia | |
dc.contributor.institution | Department of Medicine, University of Cambridge, Cambridge, UK | |
dc.identifier.volume | 7 | |
dc.identifier.issue | 6 | |
kaust.person | Abd El Ghany, Moataz | |
dc.date.accepted | 2021-03-09 | |
refterms.dateFOA | 2021-06-15T13:55:29Z | |
kaust.acknowledged.supportUnit | KAUST Bioscience Core Laboratory |
Files in this item
This item appears in the following Collection(s)
-
Articles
-
Biological and Environmental Science and Engineering (BESE) Division
For more information visit: https://bese.kaust.edu.sa/ -
Communication Theory Lab
For more information visit: https://cemse.kaust.edu.sa/ctl -
Computational Bioscience Research Center (CBRC)
-
Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division
For more information visit: https://cemse.kaust.edu.sa/