Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization
AuthorsAbd El Ghany, Moataz
Turner, A. Keith
Klena, John D.
Kingsley, Robert A.
Hill-Cawthorne, Grant A.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/669590
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AbstractEnterotoxigenic Escherichia coli (ETEC) expressing the colonization pili CFA/I are common causes of diarrhoeal infections in humans. Here, we use a combination of transposon mutagenesis and transcriptomic analysis to identify genes and pathways that contribute to ETEC persistence in water environments and colonization of a mammalian host. ETEC persisting in water exhibit a distinct RNA expression profile from those growing in richer media. Multiple pathways were identified that contribute to water survival, including lipopolysaccharide biosynthesis and stress response regulons. The analysis also indicated that ETEC growing in vivo in mice encounter a bottleneck driving down the diversity of colonizing ETEC populations.
CitationAbd El Ghany, M., Barquist, L., Clare, S., Brandt, C., Mayho, M., Joffre´, E., … Pickard, D. (2021). Functional analysis of colonization factor antigen I positive enterotoxigenic Escherichia coli identifies genes implicated in survival in water and host colonization. Microbial Genomics, 7(6). doi:10.1099/mgen.0.000554
SponsorsThe authors would like to thank KAUST Bioscience Core Laboratory for the support with generating sequencing data. The authors wish to thank the WTSI Sequencing team for their help with generating TraDIS sequencing data and the Pathogen Informatics Team for their assistance with bioinformatics analysis of TraDIS and RNAseq data.
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.
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