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dc.contributor.authorTanaka, Tsuyoshi
dc.contributor.authorMaeda, Yoshiaki
dc.contributor.authorSuhaimi, Noraiza
dc.contributor.authorTsuneoka, Chiharu
dc.contributor.authorNonoyama, Tomomi
dc.contributor.authorYoshino, Tomoko
dc.contributor.authorKato, Naohiro
dc.contributor.authorLauersen, Kyle J.
dc.date.accessioned2021-06-14T12:15:25Z
dc.date.available2021-06-14T12:15:25Z
dc.date.issued2021-05-21
dc.date.submitted2021-02-08
dc.identifier.citationTanaka, T., Maeda, Y., Suhaimi, N., Tsuneoka, C., Nonoyama, T., Yoshino, T., … Lauersen, K. J. (2021). Intron-mediated enhancement of transgene expression in the oleaginous diatom Fistulifera solaris towards bisabolene production. Algal Research, 57, 102345. doi:10.1016/j.algal.2021.102345
dc.identifier.issn2211-9264
dc.identifier.doi10.1016/j.algal.2021.102345
dc.identifier.urihttp://hdl.handle.net/10754/669573
dc.description.abstractMicroalgae have been widely recognized as promising hosts for production of various valuable compounds. However, low expression or silencing of transgenes in most microalgae species have hindered their broader implementation in biotechnological applications. In many eukaryotes, intron mediated enhancement (IME) of gene expression by inclusion of introns in heterologous sequences has resulted in a significant increase in transgene expression. This phenomenon has been found in a broad range of eukaryotic organisms including animals, plants, yeasts, and more recently green microalgae. However, no investigation has been conducted in diatoms. Here, we present findings of IME in the oleaginous diatom, Fistulifera solaris. Inclusion of a native intron found in glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene into a bleomycin-resistance gene (shble) resulted in enhancement of the mRNA level of shble, leading to increase in resistance and selection. We tested this IME in a heterologous plant sesquiterpene synthase gene construct (AgBS), leading to expression approximately double that of non-intron containing cassettes. The heterologous AgBS lead to accumulation of the sesquiterpene (E)-α-bisabolene, indicating that native precursors were amenable to manipulation and available in the cytoplasm of this diatom. To the best of our knowledge, this is the first study to investigate the effect of IME in diatoms.
dc.description.sponsorshipThis study was supported by JSPS KAKENHI Grant-in-Aid for Scientific Research B [grant number 17H03465]. The authors thank Ms. Chiharu Watanabe for microscopic observation of diatom cells.
dc.publisherElsevier BV
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S2211926421001648
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Algal Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Algal Research, [57, , (2021-05-21)] DOI: 10.1016/j.algal.2021.102345 . © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleIntron-mediated enhancement of transgene expression in the oleaginous diatom Fistulifera solaris towards bisabolene production
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering Division, King Abdullah University of Science and Technology (KAUST), Thuwal 23955-6900, Kingdom of Saudi Arabia
dc.identifier.journalAlgal Research
dc.rights.embargodate2023-05-21
dc.eprint.versionPost-print
dc.contributor.institutionDivision of Biotechnology and Life Science, Institute of Engineering, Tokyo University of Agriculture and Technology, 2-24-16, Naka-cho, Koganei, Tokyo, 184-8588, Japan
dc.contributor.institutionDepartment of Biological Sciences, Louisiana State University, Baton Rouge, LA, 70803, USA
dc.identifier.volume57
dc.identifier.pages102345
kaust.personLauersen, Kyle J.
dc.date.accepted2021-04-30
dc.identifier.eid2-s2.0-85106381353
refterms.dateFOA2021-06-15T10:30:15Z
dc.date.published-online2021-05-21
dc.date.published-print2021-07


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