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dc.contributor.authorBraguy, Justine
dc.contributor.authorSamodelov, Sophia L.
dc.contributor.authorAndres, Jennifer
dc.contributor.authorOchoa-Fernandez, Rocio
dc.contributor.authorAl-Babili, Salim
dc.contributor.authorZurbriggen, Matias D.
dc.date.accessioned2021-06-09T06:52:26Z
dc.date.available2021-06-09T06:52:26Z
dc.date.issued2021-05-25
dc.identifier.citationBraguy, J., Samodelov, S. L., Andres, J., Ochoa-Fernandez, R., Al-Babili, S., & Zurbriggen, M. D. (2021). A Protoplast-Based Bioassay to Quantify Strigolactone Activity in Arabidopsis Using StrigoQuant. Strigolactones, 201–218. doi:10.1007/978-1-0716-1429-7_16
dc.identifier.isbn9781071614280
dc.identifier.isbn9781071614297
dc.identifier.issn1940-6029
dc.identifier.issn1064-3745
dc.identifier.doi10.1007/978-1-0716-1429-7_16
dc.identifier.urihttp://hdl.handle.net/10754/669478
dc.description.abstractUnderstanding the biological background of strigolactone (SL) structural diversity and the SL signaling pathway at molecular level requires quantitative and sensitive tools that precisely determine SL dynamics. Such biosensors may be also very helpful in screening for SL analogs and mimics with defined biological functions. Recently, the genetically encoded, ratiometric sensor StrigoQuant was developed and allowed the quantification of the activity of a wide concentration range of SLs. StrigoQuant can be used for studies on the biosynthesis, function and signal transduction of this hormone class. Here, we provide a comprehensive protocol for establishing the use of StrigoQuant in Arabidopsis protoplasts. We first describe the generation and transformation of the protoplasts with StrigoQuant and detail the application of the synthetic SL analogue GR24. We then show the recording of the luminescence signal and how the obtained data are processed and used to assess/determine SL perception.
dc.description.sponsorshipThis work was supported by the baseline funding and Competitive Research Grant 4 (CRG4) given to S.A. from King Abdullah University of Science and Technology and Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy (CEPLAS—EXC-2048/—Project ID 390686111) to M.D.Z. and the iGRAD Plant (IRTG 1525) to R.O.F. and M.D.Z. We thank Maximilian Augustin and Hannes Beyer for preliminary work on the sensors.
dc.publisherSpringer US
dc.relation.urlhttps://link.springer.com/10.1007/978-1-0716-1429-7_16
dc.rightsArchived with thanks to Springer US
dc.titleA protoplast-based bioassay to quantify strigolactone activity in arabidopsis using strigoquant
dc.typeBook Chapter
dc.typeProtocol
dc.contributor.departmentPlant Science
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentDesert Agriculture Initiative
dc.rights.embargodate2022-05-25
dc.eprint.versionPost-print
dc.contributor.institutionInstitute of Synthetic Biology and CEPLAS, University of Düsseldorf, Düsseldorf, Germany
dc.contributor.institutionClinical Pharmacology and Toxicology, Universitätsspital Zürich, Zürich, Switzerland
dc.contributor.institutioniGRAD Plant Graduate School, University of Düsseldorf, Düsseldorf, Germany
dc.identifier.volume2309
dc.identifier.pages201-218
kaust.personBraguy, Justine
kaust.personAl-Babili, Salim
dc.identifier.eid2-s2.0-85107033320
refterms.dateFOA2021-06-09T10:26:25Z
kaust.acknowledged.supportUnitbaseline funding
kaust.acknowledged.supportUnitCompetitive Research Grant 4 (CRG4)


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