• Login
    View Item 
    •   Home
    • Research
    • Articles
    • View Item
    •   Home
    • Research
    • Articles
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguideTheses and Dissertations LibguideSubmit an Item

    Statistics

    Display statistics

    Polycomb-dependent differential chromatin compartmentalization determines gene coregulation in Arabidopsis

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Polycomb.pdf
    Size:
    4.760Mb
    Format:
    PDF
    Description:
    Accepted manuscript
    Download
    Type
    Article
    Authors
    Huang, Ying
    Sircar, Sanchari
    Ramirez-Prado, Juan Sebastian
    Manza-Mianza, Deborah
    Antunez-Sanchez, Javier
    Brik-Chaouche, Rim
    Rodriguez-Granados, Natalia
    An, Jing
    Bergounioux, Catherine
    Mahfouz, Magdy M. cc
    Hirt, Heribert cc
    Crespi, Martin
    Concia, Lorenzo
    Barnech, Fredy
    Amiard, Simon
    Probst, Aline V
    Gutierrez-Marcos, Jose
    Ariel, Federico
    Raynaud, Cecile
    Latrasse, David
    Benhamed, Moussa
    KAUST Department
    Bioengineering
    Biological and Environmental Science and Engineering (BESE) Division
    Center for Desert Agriculture
    Hirt Lab
    Laboratory for Genome Engineering
    Plant Science
    Date
    2021-06-03
    Online Publication Date
    2021-06-03
    Print Publication Date
    2021-07
    Embargo End Date
    2021-12-04
    Submitted Date
    2020-11-06
    Permanent link to this record
    http://hdl.handle.net/10754/669447
    
    Metadata
    Show full item record
    Abstract
    In animals, distant H3K27me3-marked Polycomb targets can establish physical interactions forming repressive chromatin hubs. In plants, growing evidence suggests that H3K27me3 act directly or indirectly to regulate chromatin interactions, although how this histone modification modulates 3D chromatin architecture remains elusive. To decipher the impact of the dynamic deposition of H3K27me3 on the Arabidopsis thaliana nuclear interactome, we combined genetics, transcriptomics and alternative 3D epigenomic approaches. By analyzing mutants defective for histone H3K27 methylation or demethylation we uncovered the crucial role of this chromatin mark in short- and previously unnoticed long-range chromatin loop formation. We found that a reduction in H3K27me3 led to a decrease in the interactions within Polycomb-associated repressive domains. Regions with lower H3K27me3 levels in the H3K27 methyltransferase clf mutant established new interactions with regions marked with H3K9ac – a histone modification associated with active transcription, thus indicating that a reduction in H3K27me3 levels induces a global reconfiguration of chromatin architecture. Altogether, our results reveal that the 3D genome organization is tightly linked to reversible histone modifications that govern chromatin interactions. Consequently, nuclear organization dynamics shapes the transcriptional reprogramming during plant development and places H3K27me3 as a key feature in the coregulation of distant genes.
    Citation
    Huang, Y., Sircar, S., Ramirez-Prado, J. S., Manza-Mianza, D., Antunez-Sanchez, J., brik-chaouche, R., … Benhamed, M. (2021). Polycomb-dependent differential chromatin compartmentalization determines gene coregulation in Arabidopsis. Genome Research, gr.273771.120. doi:10.1101/gr.273771.120
    Sponsors
    This work was supported by the Agence National de la Recherche ANR (3DWheat project ANR-19-CE20- 0001-01) and by the Institut Universitaire de France (IUF). Y.H. was supported by China Scholar Council fellowships (201806690005).
    Publisher
    Cold Spring Harbor Laboratory
    Journal
    Genome research
    DOI
    10.1101/gr.273771.120
    PubMed ID
    34083408
    Additional Links
    http://genome.cshlp.org/lookup/doi/10.1101/gr.273771.120
    ae974a485f413a2113503eed53cd6c53
    10.1101/gr.273771.120
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Center for Desert Agriculture

    entitlement

    Related articles

    • Reprogramming of H3K27me3 is critical for acquisition of pluripotency from cultured Arabidopsis tissues.
    • Authors: He C, Chen X, Huang H, Xu L
    • Issue date: 2012 Aug
    • A novel bivalent chromatin associates with rapid induction of camalexin biosynthesis genes in response to a pathogen signal in <i>Arabidopsis</i>.
    • Authors: Zhao K, Kong D, Jin B, Smolke CD, Rhee SY
    • Issue date: 2021 Sep 15
    • The CHD3 remodeler PICKLE associates with genes enriched for trimethylation of histone H3 lysine 27.
    • Authors: Zhang H, Bishop B, Ringenberg W, Muir WM, Ogas J
    • Issue date: 2012 May
    • PRC2-mediated H3K27me3 modulates shoot iron homeostasis in <i>Arabidopsis thaliana</i>.
    • Authors: Park EY, Tsuyuki KM, Parsons EM, Jeong J
    • Issue date: 2020 Sep 1
    • Epigenomic Regulation of Schwann Cell Reprogramming in Peripheral Nerve Injury.
    • Authors: Ma KH, Hung HA, Svaren J
    • Issue date: 2016 Aug 31
    DSpace software copyright © 2002-2022  DuraSpace
    Quick Guide | Contact Us | KAUST University Library
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.