Polycomb-dependent differential chromatin compartmentalization determines gene coregulation in Arabidopsis
Ramirez-Prado, Juan Sebastian
Mahfouz, Magdy M.
Probst, Aline V
Biological and Environmental Science and Engineering (BESE) Division
Center for Desert Agriculture
Laboratory for Genome Engineering
Online Publication Date2021-06-03
Print Publication Date2021-07
Embargo End Date2021-12-04
Permanent link to this recordhttp://hdl.handle.net/10754/669447
MetadataShow full item record
AbstractIn animals, distant H3K27me3-marked Polycomb targets can establish physical interactions forming repressive chromatin hubs. In plants, growing evidence suggests that H3K27me3 act directly or indirectly to regulate chromatin interactions, although how this histone modification modulates 3D chromatin architecture remains elusive. To decipher the impact of the dynamic deposition of H3K27me3 on the Arabidopsis thaliana nuclear interactome, we combined genetics, transcriptomics and alternative 3D epigenomic approaches. By analyzing mutants defective for histone H3K27 methylation or demethylation we uncovered the crucial role of this chromatin mark in short- and previously unnoticed long-range chromatin loop formation. We found that a reduction in H3K27me3 led to a decrease in the interactions within Polycomb-associated repressive domains. Regions with lower H3K27me3 levels in the H3K27 methyltransferase clf mutant established new interactions with regions marked with H3K9ac – a histone modification associated with active transcription, thus indicating that a reduction in H3K27me3 levels induces a global reconfiguration of chromatin architecture. Altogether, our results reveal that the 3D genome organization is tightly linked to reversible histone modifications that govern chromatin interactions. Consequently, nuclear organization dynamics shapes the transcriptional reprogramming during plant development and places H3K27me3 as a key feature in the coregulation of distant genes.
CitationHuang, Y., Sircar, S., Ramirez-Prado, J. S., Manza-Mianza, D., Antunez-Sanchez, J., brik-chaouche, R., … Benhamed, M. (2021). Polycomb-dependent differential chromatin compartmentalization determines gene coregulation in Arabidopsis. Genome Research, gr.273771.120. doi:10.1101/gr.273771.120
SponsorsThis work was supported by the Agence National de la Recherche ANR (3DWheat project ANR-19-CE20- 0001-01) and by the Institut Universitaire de France (IUF). Y.H. was supported by China Scholar Council fellowships (201806690005).
PublisherCold Spring Harbor Laboratory
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