Polycomb-dependent differential chromatin compartmentalization determines gene coregulation in Arabidopsis
Type
ArticleAuthors
Huang, YingSircar, Sanchari
Ramirez-Prado, Juan Sebastian
Manza-Mianza, Deborah
Antunez-Sanchez, Javier
Brik-Chaouche, Rim
Rodriguez-Granados, Natalia
An, Jing
Bergounioux, Catherine
Mahfouz, Magdy M.

Hirt, Heribert

Crespi, Martin
Concia, Lorenzo
Barnech, Fredy
Amiard, Simon
Probst, Aline V
Gutierrez-Marcos, Jose
Ariel, Federico
Raynaud, Cecile
Latrasse, David
Benhamed, Moussa
KAUST Department
BioengineeringBiological and Environmental Science and Engineering (BESE) Division
Center for Desert Agriculture
Hirt Lab
Laboratory for Genome Engineering
Plant Science
Date
2021-06-03Online Publication Date
2021-06-03Print Publication Date
2021-07Embargo End Date
2021-12-04Submitted Date
2020-11-06Permanent link to this record
http://hdl.handle.net/10754/669447
Metadata
Show full item recordAbstract
In animals, distant H3K27me3-marked Polycomb targets can establish physical interactions forming repressive chromatin hubs. In plants, growing evidence suggests that H3K27me3 act directly or indirectly to regulate chromatin interactions, although how this histone modification modulates 3D chromatin architecture remains elusive. To decipher the impact of the dynamic deposition of H3K27me3 on the Arabidopsis thaliana nuclear interactome, we combined genetics, transcriptomics and alternative 3D epigenomic approaches. By analyzing mutants defective for histone H3K27 methylation or demethylation we uncovered the crucial role of this chromatin mark in short- and previously unnoticed long-range chromatin loop formation. We found that a reduction in H3K27me3 led to a decrease in the interactions within Polycomb-associated repressive domains. Regions with lower H3K27me3 levels in the H3K27 methyltransferase clf mutant established new interactions with regions marked with H3K9ac – a histone modification associated with active transcription, thus indicating that a reduction in H3K27me3 levels induces a global reconfiguration of chromatin architecture. Altogether, our results reveal that the 3D genome organization is tightly linked to reversible histone modifications that govern chromatin interactions. Consequently, nuclear organization dynamics shapes the transcriptional reprogramming during plant development and places H3K27me3 as a key feature in the coregulation of distant genes.Citation
Huang, Y., Sircar, S., Ramirez-Prado, J. S., Manza-Mianza, D., Antunez-Sanchez, J., brik-chaouche, R., … Benhamed, M. (2021). Polycomb-dependent differential chromatin compartmentalization determines gene coregulation in Arabidopsis. Genome Research, gr.273771.120. doi:10.1101/gr.273771.120Sponsors
This work was supported by the Agence National de la Recherche ANR (3DWheat project ANR-19-CE20- 0001-01) and by the Institut Universitaire de France (IUF). Y.H. was supported by China Scholar Council fellowships (201806690005).Publisher
Cold Spring Harbor LaboratoryJournal
Genome researchPubMed ID
34083408Additional Links
http://genome.cshlp.org/lookup/doi/10.1101/gr.273771.120ae974a485f413a2113503eed53cd6c53
10.1101/gr.273771.120
Scopus Count
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