Undercover Toxic Ménage à Trois of Amylin, Copper (II) and Metformin in Human Embryonic Kidney Cells

Abstract
In recent decades, type 2 diabetes complications have been correlated with amylin aggregation, copper homeostasis and metformin side effects. However, each factor was analyzed separately, and only in some rare cases copper/amylin or copper/metformin complexes were considered. We demonstrate for the first time that binary metformin/amylin and tertiary copper (II)/amylin/metformin complexes of high cellular toxicity are formed and lead to the formation of aggregated multi-level lamellar structures on the cell membrane. Considering the increased concentration of amylin, copper (II) and metformin in kidneys of T2DM patients, our findings on the toxicity of amylin and its adducts may be correlated with diabetic nephropathy development.

Citation
Congiu, T., Alghrably, M. N., Emwas, A.-H., Jaremko, L., Lachowicz, J. I., Piludu, M., … Coni, P. (2021). Undercover Toxic Ménage à Trois of Amylin, Copper (II) and Metformin in Human Embryonic Kidney Cells. Pharmaceutics, 13(6), 830. doi:10.3390/pharmaceutics13060830

Acknowledgements
This research was partially funded by FIR 2020 and from Regione Autonoma della Sardegna (grant RASSR79857).

Publisher
MDPI AG

Journal
Pharmaceutics

DOI
10.3390/pharmaceutics13060830

Additional Links
https://www.mdpi.com/1999-4923/13/6/830

Permanent link to this record