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dc.contributor.authorOtterbach, Sophie L.
dc.contributor.authorKhoury, Holly
dc.contributor.authorRupasinghe, Thusitha
dc.contributor.authorMendis, Himasha
dc.contributor.authorKwan, Kim H.
dc.contributor.authorLui, Veronica
dc.contributor.authorNatera, Siria H. A.
dc.contributor.authorKlaiber, Iris
dc.contributor.authorAllen, Nathaniel M.
dc.contributor.authorJarvis, David E.
dc.contributor.authorTester, Mark
dc.contributor.authorRoessner, Ute
dc.contributor.authorSchmoeckel, Sandra Manuela
dc.date.accessioned2021-09-13T08:46:54Z
dc.date.available2021-05-26T07:40:56Z
dc.date.available2021-09-13T08:46:54Z
dc.date.issued2021-09-12
dc.date.submitted2021-04-30
dc.identifier.citationOtterbach, S. L., Khoury, H., Rupasinghe, T., Mendis, H., Kwan, K. H., Lui, V., … Schmöckel, S. M. (2021). Characterization of Epidermal Bladder Cells in Chenopodium quinoa. Plant, Cell & Environment. doi:10.1111/pce.14181
dc.identifier.issn0140-7791
dc.identifier.issn1365-3040
dc.identifier.doi10.1111/pce.14181
dc.identifier.urihttp://hdl.handle.net/10754/669252
dc.description.abstractChenopodium quinoa (quinoa) is considered a superfood with its favourable nutrient composition and being gluten free. Quinoa has high tolerance to abiotic stresses, such as salinity, water deficit (drought) and cold. The tolerance mechanisms are yet to be elucidated. Quinoa has Epidermal Bladder Cells (EBCs) that densely cover the shoot surface, particularly the younger parts of the plant. Here, we report on the EBC's primary and secondary metabolomes, as well as the lipidome in control conditions and in response to abiotic stresses. EBCs were isolated from plants after cold, heat, high-light, water deficit and salt treatments. We used untargeted Gas Chromatography-Mass Spectrometry (GC-MS) to analyse metabolites and untargeted and targeted Liquid Chromatography-MS (LC-MS) for lipids and secondary metabolite analyses. We identified 64 primary metabolites, including sugars, organic acids and amino acids, 19 secondary metabolites, including phenolic compounds, betanin and saponins and 240 lipids categorized in five groups including glycerolipids and phospholipids. We found only few changes in the metabolic composition of EBCs in response to abiotic stresses, these were metabolites related with heat, cold and high-light treatments, but not salt stress. Na+ concentrations were low in EBCs with all treatments, and approximately two orders of magnitude lower than K+ concentrations.
dc.description.sponsorshipHK was supported by King Abdullah University of Science and Technology (VSRP funding). SMS received funding from the Ministry for Science, Research and Art of Baden-Wuerttemberg, Germany (Az: 75533-30-20/1). Lipid and primary metabolite analyses were performed at Metabolomics Australia at University of Melbourne, which is a National Collaborative Research Infrastructure Strategy initiative under Bioplatforms Australia Pty Ltd (http://www.bioplatforms.com/). We thank Noha O. Saber (KAUST, Saudi Arabia) for technical assistance, Alexa Wangler und Pascal Wendel (BSc, UHOH, Germany) for help with EBC collections. All authors declare no conflict of interest.
dc.publisherWiley
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/10.1111/pce.14181
dc.rightsArchived with thanks to Plant, Cell & Environment
dc.subjectChenopodium quinoa
dc.subjectepidermal bladder cells
dc.subjectEBC
dc.subjectabiotic stress
dc.subjectmetabolomics
dc.subjectlipidomics
dc.titleCharacterization of Epidermal Bladder Cells in Chenopodium quinoa
dc.typeArticle
dc.contributor.departmentKing Abdullah University of Science and Technology Thuwal Saudi Arabia
dc.identifier.journalPlant, Cell & Environment
dc.rights.embargodate2022-09-12
dc.eprint.versionPost-print
dc.contributor.institutionDepartment of Crop Science, Faculty of Agriculture University of Hohenheim Stuttgart Germany
dc.contributor.institutionSchool of BioSciences The University of Melbourne Victoria Australia
dc.contributor.institutionMetabolomics Australia The University of Melbourne Victoria Australia
dc.contributor.institutionCore Facility Hohenheim (640). Mass Spectrometry Unit University of Hohenheim Stuttgart Germany
dc.contributor.institutionDepartment of Plant and Wildlife Sciences, College of Life Sciences Brigham Young University Provo Utah USA
kaust.personKlaiber, Iris
kaust.grant.numberNoha O. Saber
dc.date.accepted2021-08-23
refterms.dateFOA2021-05-26T07:41:56Z
kaust.acknowledged.supportUnitVSRP


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