Characterization of Epidermal Bladder Cells in Chenopodium quinoa
Tester, Mark A.
KAUST DepartmentPlant Science
Desert Agriculture Initiative
Biological and Environmental Sciences and Engineering (BESE) Division
KAUST Grant NumberNoha O. Saber
Permanent link to this recordhttp://hdl.handle.net/10754/669252
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AbstractChenopodium quinoa (quinoa) is considered a superfood, as it has favourable nutrient composition and is gluten free. Quinoa has high tolerance to several abiotic stresses, i.e. salinity, water deficit (drought) and cold. The tolerance mechanisms are yet to be elucidated. Quinoa has Epidermal Bladder Cells (EBCs) that densely cover the shoot surface, particularly the younger parts of the plant. Here, we report on the EBC’s primary and secondary metabolomes, as well as the lipidome in response to abiotic stresses. EBCs were isolated from plants after cold, heat, high-light, water deficit and salt treatments. We used untargeted Gas Chromatography-Mass Spectrometry (GC-MS) to analyse metabolites and untargeted and targeted Liquid Chromatography-MS (LC-MS) for lipids and secondary metabolite analyses. We identified 64 primary metabolites, including sugars, organic acids and amino acids, 19 secondary metabolites, including phenolic compounds, betanin and saponins and 240 lipids categorized in five groups including glycerolipids and phospholipids. Although we found only few changes in the metabolic composition of bladders in response to abiotic stresses, metabolites related with heat, cold and high-light treatments, but not salt stress, were changed significantly. Na+ concentrations were low in EBCs with all treatments, and approximately two orders of magnitude lower than K+ concentrations.
CitationOtterbach, S., Khoury, H., Rupasinghe, T., Mendis, H., Kwan, K., Lui, V., … Schmöckel, S. (2021). Characterization of Epidermal Bladder Cells in Chenopodium quinoa. doi:10.22541/au.162063490.09509555/v1
SponsorsHK was supported by King Abdullah University of Science and Technology (VSRP funding). SMS received funding from the Ministry for Science, Research and Art of Baden-Wuerttemberg, Germany (Az: 75533-30-20/1). Lipid and primary metabolite analyses were performed at Metabolomics Australia at University of Melbourne, which is a National Collaborative Research Infrastructure Strategy initiative under Bioplatforms Australia Pty Ltd (http://www.bioplatforms.com/). We thank Noha O. Saber (KAUST, Saudi Arabia) for technical assistance, Alexa Wangler und Pascal Wendel (BSc, UHOH, Germany) for help with bladder collections. All authors declare no conflict of interest