The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis
van der Wal, Froukje
Suresh Devani, Ravi
Immink, Richard G. H.
KAUST DepartmentPlant Science
Desert Agriculture Initiative
Biological and Environmental Sciences and Engineering (BESE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/669225
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AbstractHEAT SHOCK FACTOR A2 (HSFA2) is a regulator of multiple environmental stress responses required for stress acclimation. We analyzed HSFA2 co-regulated genes and identified 43 genes strongly co-regulated with HSFA2 during multiple stresses. Motif enrichment analysis revealed an over-representation of the site II element (SIIE) in the promoters of these genes. In a yeast 1-hybrid screen with the SIIE, we identified the closely related R2R3-MYB transcription factors TT2 and MYB5. We found overexpression of MYB5 or TT2 rendered plants heat stress tolerant. In contrast, tt2, myb5, and tt2/myb5 loss of function mutants showed heat stress hypersensitivity. Transient expression assays confirmed that MYB5 and TT2 can regulate the HSFA2 promoter together with the other members of the MBW complex, TT8 and TRANSPARENT TESTA GLABRA 1 (TTG1) and that the SIIE was involved in this regulation. Transcriptomic analysis revealed that TT2/MYB5 target promoters were enriched in SIIE. Overall, we report a new function of TT2 and MYB5 in stress resistance and a role in SIIE-mediated HSFA2 regulation.
CitationJacob, P., Brisou, G., Dalmais, M., Thévenin, J., van der Wal, F., Latrasse, D., … Bendahmane, A. (2021). The Seed Development Factors TT2 and MYB5 Regulate Heat Stress Response in Arabidopsis. Genes, 12(5), 746. doi:10.3390/genes12050746
SponsorsWe thank P. Audigier for plant handling, and are grateful for the cell biology imaging platform and research facilities provided by the Institute of Plant-Science Paris-Saclay (IPS2).
Except where otherwise noted, this item's license is described as Archived with thanks to Genes. © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).