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dc.contributor.authorVallarino, José G
dc.contributor.authorMi, Jianing
dc.contributor.authorPetřík, Ivan
dc.contributor.authorNovak, Ondrej
dc.contributor.authorCorrea, Sandra Marcela
dc.contributor.authorKosmacz, Monika
dc.contributor.authorHavaux, Michel
dc.contributor.authorRodriguez-Concepcion, Manuel
dc.contributor.authorAl-Babili, Salim
dc.contributor.authorFernie, Alisdair R.
dc.contributor.authorSkirycz, Aleksandra
dc.contributor.authorMoreno, Juan C
dc.identifier.citationVallarino, J. G., Mi, J., Petřík, I., Novak, O., Correa, S. M., Kosmacz, M., … Moreno Beltran, J. C. (2021). Manipulation of carotenoid metabolism stimulates biomass and stress tolerance in tomato. doi:10.1101/2021.05.05.442770
dc.description.abstractImproving yield, nutritional value and tolerance to abiotic stress are major targets of current breeding and biotechnological approaches that aim at increasing crop production and ensuring food security. Metabolic engineering of carotenoids, the precursor of Vitamin-A and plant hormones that regulate plant growth and response to adverse growth conditions, has been mainly focusing on provitamin A biofortification or the production of high-value carotenoids. Here, we show that the introduction of a single gene of the carotenoid biosynthetic pathway in different tomato cultivars simultaneously improved photosynthetic capacity and tolerance to various abiotic stresses (e.g., high light, salt, and drought), caused an up to 77% fruit yield increase and enhanced fruit's provitamin A content and shelf life. Our findings pave the way for developing a new generation of crops that combine high productivity and increased nutritional value with the capability to cope with climate change-related environmental challenges.
dc.description.sponsorshipWe are grateful to Prof. Dr. Lothar Willmitzer for his support and advice. We thank Prof. Dr. Ralph Bock (Max Planck Institute of Molecular Plant physiology, Golm, Germany) and Dr. Caterina D’Ambrosio (Centro Ricerche Metapontum Agrobios, ALSIA, Italy) for kindly providing the transplastomic pNLyc#2 and LCe seeds and the homozygous nuclear High Caro (H.C.) lines, respectively. We thank Dr. Camila Caldana and Anne Michaelis for providing the GC facility and running the GC samples, respectively, and Maria Rosa Rodriguez-Goberna for technical support related with pigment analysis (supported by grant BIO2017-84041-P from the Spanish AEI). In addition, we thank Hana Martínková and Petra Amakorová for their help with phytohormone analyses. The hormonomics work was funded by the Ministry of Education, Youth and Sports of the Czech Republic (European Regional Development Fund-Project “Plants as a tool for sustainable global development” No. CZ.02.1.01/0.0/0.0/16_019/0000827), and the Internal Grant Agency of Palacký University (IGA_PrF_2021_011)
dc.publisherCold Spring Harbor Laboratory
dc.rightsThe copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuit.
dc.titleManipulation of carotenoid metabolism stimulates biomass and stress tolerance in tomato
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentCenter for Desert Agriculture
dc.contributor.departmentPlant Science
dc.contributor.institutionMax Planck Institut für Molekulare Pflanzenphysiologie, Am Mühlenberg1 D-14476, Potsdam6 Golm, Germany.
dc.contributor.institutionLaboratory of Growth Regulators, Faculty of Science, Palacký University and Institute of Experimental Botany, The Czech Academy of Sciences, Šlechtitelů 27, CZ-78371 Olomouc, Czech Republic.
dc.contributor.institutionAix-Marseille University, CEA, CNRS UMR7265, BIAM, CEA/Cadarache, F-13108 Saint-Paul13 lez-Durance, France.
dc.contributor.institutionInstitute for Plant Molecular and Cell Biology (IBMCP) UPV-CSIC, 46022 Valencia, Spain.
dc.contributor.institutionBoyce Thompson Institute, Cornell University, Ithaca, NY, United States.
kaust.personMi, Jianing
kaust.personKosmacz, Monika
kaust.personAl-Babili, Salim
kaust.personMoreno Beltran, Juan Camilo

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The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuit.
Except where otherwise noted, this item's license is described as The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuit.