Alternative glycosylation controls endoplasmic reticulum dynamics and tubular extension in mammalian cells
dc.contributor.author | Kerselidou, Despoina | |
dc.contributor.author | Dohai, Bushra Saeed | |
dc.contributor.author | Nelson, David R. | |
dc.contributor.author | Daakour, Sarah | |
dc.contributor.author | De Cock, Nicolas | |
dc.contributor.author | Hassoun, Zahra Al Oula | |
dc.contributor.author | Kim, Dae-Kyum | |
dc.contributor.author | Olivet, Julien | |
dc.contributor.author | El Assal, Diana C. | |
dc.contributor.author | Jaiswal, Ashish | |
dc.contributor.author | Alzahmi, Amnah | |
dc.contributor.author | Saha, Deeya | |
dc.contributor.author | Pain, Charlotte | |
dc.contributor.author | Matthijssens, Filip | |
dc.contributor.author | Lemaitre, Pierre | |
dc.contributor.author | Herfs, Michael | |
dc.contributor.author | Chapuis, Julien | |
dc.contributor.author | Ghesquiere, Bart | |
dc.contributor.author | Vertommen, Didier | |
dc.contributor.author | Kriechbaumer, Verena | |
dc.contributor.author | Knoops, Kèvin | |
dc.contributor.author | Lopez-Iglesias, Carmen | |
dc.contributor.author | van Zandvoort, Marc | |
dc.contributor.author | Lambert, Jean-Charles | |
dc.contributor.author | Hanson, Julien | |
dc.contributor.author | Desmet, Christophe | |
dc.contributor.author | Thiry, Marc | |
dc.contributor.author | Lauersen, Kyle J. | |
dc.contributor.author | Vidal, Marc | |
dc.contributor.author | Van Vlierberghe, Pieter | |
dc.contributor.author | Dequiedt, Franck | |
dc.contributor.author | Salehi-Ashtiani, Kourosh | |
dc.contributor.author | Twizere, Jean-Claude | |
dc.date.accessioned | 2021-05-11T09:21:15Z | |
dc.date.available | 2021-05-11T09:21:15Z | |
dc.date.issued | 2021-05-07 | |
dc.date.submitted | 2020-09-27 | |
dc.identifier.citation | Kerselidou, D., Dohai, B. S., Nelson, D. R., Daakour, S., De Cock, N., Hassoun, Z. A. O., … Twizere, J.-C. (2021). Alternative glycosylation controls endoplasmic reticulum dynamics and tubular extension in mammalian cells. Science Advances, 7(19), eabe8349. doi:10.1126/sciadv.abe8349 | |
dc.identifier.issn | 2375-2548 | |
dc.identifier.pmid | 33962942 | |
dc.identifier.doi | 10.1126/sciadv.abe8349 | |
dc.identifier.uri | http://hdl.handle.net/10754/669164 | |
dc.description.abstract | The endoplasmic reticulum (ER) is a central eukaryotic organelle with a tubular network made of hairpin proteins linked by hydrolysis of guanosine triphosphate nucleotides. Among posttranslational modifications initiated at the ER level, glycosylation is the most common reaction. However, our understanding of the impact of glycosylation on the ER structure remains unclear. Here, we show that exostosin-1 (EXT1) glycosyltransferase, an enzyme involved in N-glycosylation, is a key regulator of ER morphology and dynamics. We have integrated multiomics and superresolution imaging to characterize the broad effect of EXT1 inactivation, including the ER shape-dynamics-function relationships in mammalian cells. We have observed that inactivating EXT1 induces cell enlargement and enhances metabolic switches such as protein secretion. In particular, suppressing EXT1 in mouse thymocytes causes developmental dysfunctions associated with the ER network extension. Last, our data illuminate the physical and functional aspects of the ER proteome-glycome-lipidome structure axis, with implications in biotechnology and medicine. | |
dc.description.sponsorship | D.-K.K. was supported by Banting Postdoctoral Fellowship of Canada and Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2017R1A6A3A03004385). B.S.D., S.D., D.R.N., A.J., D.C.E.A., and K.S.-A. were supported by New York University Abu Dhabi (NYUAD) Institute grant 73 71210 CGSB9 and NYUAD Faculty Research Fund AD060. D.K. was supported by an FRS-FNRS-Télévie Fellowship no. 7651317F (J.-C.T.). J.-C.T. is a Maitre de Recherche of the FRS-FNRS. Primarily, the Fonds de la Recherche Scientifique (FRS-FNRS) and the Fonds Leon Fredericq grants supported this work. | |
dc.publisher | American Association for the Advancement of Science (AAAS) | |
dc.relation.url | https://advances.sciencemag.org/lookup/doi/10.1126/sciadv.abe8349 | |
dc.rights | Exclusive licensee American Association for the Advancement of Science. No claim to original U.S.Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Alternative glycosylation controls endoplasmic reticulum dynamics and tubular extension in mammalian cells | |
dc.type | Article | |
dc.contributor.department | Biological and Environmental Science and Engineering (BESE) Division | |
dc.contributor.department | Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia. | |
dc.identifier.journal | Science Advances | |
dc.eprint.version | Publisher's Version/PDF | |
dc.contributor.institution | Laboratory of Viral Interactomes, GIGA Institute, University of Liege, Liege, Belgium. | |
dc.contributor.institution | Laboratory of Gene expression and Cancer, GIGA Institute, University of Liege, Liege, Belgium. | |
dc.contributor.institution | Division of Science and Math, New York University Abu Dhabi, Abu Dhabi, UAE. | |
dc.contributor.institution | Center for Genomics and Systems Biology (CGSB), New York University Abu Dhabi, Abu Dhabi, UAE. | |
dc.contributor.institution | TERRA Teaching and Research Centre, University of Liege, Liege, Belgium. | |
dc.contributor.institution | The Donnelly Centre, University of Toronto, Toronto, ON, Canada. | |
dc.contributor.institution | Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada. | |
dc.contributor.institution | Lunenfeld Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada. | |
dc.contributor.institution | Plant Cell Biology, Biological and Medical Sciences, Oxford Brookes University, Oxford, UK. | |
dc.contributor.institution | Department of Biomolecular Medicine and Center for Medical Genetics, Ghent University, Ghent, Belgium. | |
dc.contributor.institution | Cancer Research Institute Ghent (CRIG), Ghent, Belgium. | |
dc.contributor.institution | GIGA-I3 Unit, GIGA Institute, University of Liege, Liege, Belgium. | |
dc.contributor.institution | GIGA-Cancer Unit, GIGA Institute, University of Liege, Liege, Belgium. | |
dc.contributor.institution | Laboratory of Excellence Distalz, INSERM Unit 1167, Pasteur Institute of Lille, Lille, France. | |
dc.contributor.institution | Metabolomics Expertise Center, Center for Cancer Biology, VIB Center for Cancer Biology, Leuven, Belgium. | |
dc.contributor.institution | de Duve Institute, Catholic University of Louvain, Brussels, Belgium. | |
dc.contributor.institution | Microscopy CORE Lab, Maastricht Multimodal Molecular Imaging Institute, Maastricht University, Maastricht, Netherlands. | |
dc.contributor.institution | Department of Cell Biology, School for Cardiovascular Diseases (CARIM), School for Nutrition and Translational Research in Metabolism (NUTRIM), School for Mental health and Neuroscience (MHeNS), and School for Oncology and Developmental Biology (GROW), Maastricht University, Maastricht, Netherlands. | |
dc.contributor.institution | GIGA-Molecular Pharmacology, University of Liege, Liege, Belgium. | |
dc.contributor.institution | Laboratory of cell and tissue Biology, GIGA-Neurosciences, University of Liege, Liege, Belgium. | |
dc.contributor.institution | Center for Cancer Systems Biology (CCSB), Dana-Farber Cancer Institute, Boston, MA, USA. | |
dc.contributor.institution | Department of Genetics, Blavatnik Institute, Harvard Medical School, Boston, MA, USA. | |
dc.identifier.volume | 7 | |
dc.identifier.issue | 19 | |
dc.identifier.pages | eabe8349 | |
kaust.person | Lauersen, Kyle J. | |
dc.date.accepted | 2021-03-18 | |
refterms.dateFOA | 2021-05-11T09:29:02Z | |
dc.date.published-online | 2021-05-07 | |
dc.date.published-print | 2021-05 |
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