Scaffolds from Self-Assembling Tetrapeptides Support 3D Spreading, Osteogenic Differentiation, and Angiogenesis of Mesenchymal Stem Cells
KAUST DepartmentBioscience Program
Biological and Environmental Sciences and Engineering (BESE) Division
Computational Bioscience Research Center (CBRC)
Embargo End Date2022-04-28
Permanent link to this recordhttp://hdl.handle.net/10754/669066
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AbstractThe apparent rise of bone disorders demands advanced treatment protocols involving tissue engineering. Here, we describe self-assembling tetrapeptide scaffolds for the growth and osteogenic differentiation of human mesenchymal stem cells (hMSCs). The rationally designed peptides are synthetic amphiphilic self-assembling peptides composed of four amino acids that are nontoxic. These tetrapeptides can quickly solidify to nanofibrous hydrogels that resemble the extracellular matrix and provide a three-dimensional (3D) environment for cells with suitable mechanical properties. Furthermore, we can easily tune the stiffness of these peptide hydrogels by just increasing the peptide concentration, thus providing a wide range of peptide hydrogels with different stiffnesses for 3D cell culture applications. Since successful bone regeneration requires both osteogenesis and vascularization, our scaffold was found to be able to promote angiogenesis of human umbilical vein endothelial cells (HUVECs) in vitro. The results presented suggest that ultrashort peptide hydrogels are promising candidates for applications in bone tissue engineering.
CitationAlshehri, S., Susapto, H. H., & Hauser, C. A. E. (2021). Scaffolds from Self-Assembling Tetrapeptides Support 3D Spreading, Osteogenic Differentiation, and Angiogenesis of Mesenchymal Stem Cells. Biomacromolecules. doi:10.1021/acs.biomac.1c00205
SponsorsThis work was financially supported by the King Abdullah University of Science and Technology. The authors acknowledge Dr. Abdul-Hamid Emwas for NMR support. The authors acknowledge Sarah Ghalayini for proofreading. The graphical abstract was created with BioRender.com.
PublisherAmerican Chemical Society (ACS)
Except where otherwise noted, this item's license is described as https://creativecommons.org/licenses/by/4.0/