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dc.contributor.authorMarondedze, Claudius
dc.contributor.authorWong, Aloysius Tze
dc.contributor.authorThomas, Ludivine
dc.contributor.authorIrving, Helen
dc.contributor.authorGehring, Christoph A
dc.date.accessioned2021-04-14T06:24:03Z
dc.date.available2021-04-14T06:24:03Z
dc.date.issued2016-01-01
dc.identifier.citationMarondedze, C., Wong, A., Thomas, L., Irving, H., & Gehring, C. (2015). Cyclic Nucleotide Monophosphates in Plants and Plant Signaling. Handbook of Experimental Pharmacology, 87–103. doi:10.1007/164_2015_35
dc.identifier.isbn9783319526713
dc.identifier.isbn9783319526737
dc.identifier.issn0171-2004
dc.identifier.issn1865-0325
dc.identifier.pmid26721677
dc.identifier.doi10.1007/164_2015_35
dc.identifier.urihttp://hdl.handle.net/10754/668736
dc.description.abstractCyclic nucleotide monophosphates (cNMPs) and the enzymes that can generate them are of increasing interest in the plant sciences. Arguably, the major recent advance came with the release of the complete Arabidopsis thaliana genome that has enabled the systematic search for adenylate (ACs) or guanylate cyclases (GCs) and did eventually lead to the discovery of a number of GCs in higher plants. Many of these proteins have complex domain architectures with AC or GC centers moonlighting within cytosolic kinase domains. Recent reports indicated the presence of not just the canonical cNMPs (i.e., cAMP and cGMP), but also the noncanonical cCMP, cUMP, cIMP, and cdTMP in plant tissues, and this raises several questions. Firstly, what are the functions of these cNMPs, and, secondly, which enzymes can convert the substrate triphosphates into the respective noncanonical cNMPs? The first question is addressed here by comparing the reactive oxygen species (ROS) response of cAMP and cGMP to that elicited by the noncanonical cCMP or cIMP. The results show that particularly cIMP can induce significant ROS production. To answer, at least in part, the second question, we have evaluated homology models of experimentally confirmed plant GCs probing the substrate specificity by molecular docking simulations to determine if they can conceivably catalytically convert substrates other than ATP or GTP. In summary, molecular modeling and substrate docking simulations can contribute to the evaluation of cyclases for noncanonical cyclic mononucleotides and thereby further our understanding of the molecular mechanism that underlie cNMP-dependent signaling in planta.
dc.publisherSpringer Nature
dc.relation.urlhttps://link.springer.com/chapter/10.1007%2F164_2015_35
dc.rightsArchived with thanks to Springer International Publishing
dc.titleCyclic Nucleotide Monophosphates in Plants and Plant Signaling
dc.typeBook Chapter
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentBioscience Core Lab
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputer, Electrical and Mathematical Science and Engineering (CEMSE) Division
dc.contributor.departmentMolecular Signalling Group
dc.eprint.versionPost-print
dc.contributor.institutionDepartment of Biochemistry, Cambridge Centre for Proteomics, University of Cambridge, Cambridge, United Kingdom
dc.contributor.institutionDrug Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash University, Melbourne, Australia
dc.identifier.volume238
dc.identifier.pages87-103
kaust.personWong, Aloysius Tze
kaust.personThomas, Ludivine
kaust.personGehring, Christoph A.
dc.identifier.eid2-s2.0-85018952716


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