Type
Book ChapterKAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionBioscience Core Lab
Bioscience Program
Computational Bioscience Research Center (CBRC)
Computer, Electrical and Mathematical Science and Engineering (CEMSE) Division
Molecular Signalling Group
Date
2016-01-01Permanent link to this record
http://hdl.handle.net/10754/668736
Metadata
Show full item recordAbstract
Cyclic nucleotide monophosphates (cNMPs) and the enzymes that can generate them are of increasing interest in the plant sciences. Arguably, the major recent advance came with the release of the complete Arabidopsis thaliana genome that has enabled the systematic search for adenylate (ACs) or guanylate cyclases (GCs) and did eventually lead to the discovery of a number of GCs in higher plants. Many of these proteins have complex domain architectures with AC or GC centers moonlighting within cytosolic kinase domains. Recent reports indicated the presence of not just the canonical cNMPs (i.e., cAMP and cGMP), but also the noncanonical cCMP, cUMP, cIMP, and cdTMP in plant tissues, and this raises several questions. Firstly, what are the functions of these cNMPs, and, secondly, which enzymes can convert the substrate triphosphates into the respective noncanonical cNMPs? The first question is addressed here by comparing the reactive oxygen species (ROS) response of cAMP and cGMP to that elicited by the noncanonical cCMP or cIMP. The results show that particularly cIMP can induce significant ROS production. To answer, at least in part, the second question, we have evaluated homology models of experimentally confirmed plant GCs probing the substrate specificity by molecular docking simulations to determine if they can conceivably catalytically convert substrates other than ATP or GTP. In summary, molecular modeling and substrate docking simulations can contribute to the evaluation of cyclases for noncanonical cyclic mononucleotides and thereby further our understanding of the molecular mechanism that underlie cNMP-dependent signaling in planta.Citation
Marondedze, C., Wong, A., Thomas, L., Irving, H., & Gehring, C. (2015). Cyclic Nucleotide Monophosphates in Plants and Plant Signaling. Handbook of Experimental Pharmacology, 87–103. doi:10.1007/164_2015_35Publisher
Springer NatureISBN
97833195267139783319526737
PubMed ID
26721677Additional Links
https://link.springer.com/chapter/10.1007%2F164_2015_35ae974a485f413a2113503eed53cd6c53
10.1007/164_2015_35
Scopus Count
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