• Login
    View Item 
    •   Home
    • Research
    • Conference Papers
    • View Item
    •   Home
    • Research
    • Conference Papers
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguideTheses and Dissertations LibguideSubmit an Item

    Statistics

    Display statistics

    Time-dependent pulsing of microfluidic pumps to enhance 3D bioprinting of peptide bioinks

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Thumbnail
    Name:
    Conference Paperfile1.pdf
    Size:
    1.241Mb
    Format:
    PDF
    Description:
    publisher's version
    Download
    Type
    Conference Paper
    Presentation
    Authors
    Khan, Zainab
    Kahin, Kowther
    Hauser, Charlotte cc
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Bioscience Program
    Computational Bioscience Research Center (CBRC)
    Department of Biological and Environmental Science and Engineering, King Abdullah University for Science and Technology, Thuwal, 23955-6900, Saudi Arabia
    Environmental Science and Engineering Program
    Date
    2021-03-05
    Permanent link to this record
    http://hdl.handle.net/10754/668732
    
    Metadata
    Show full item record
    Abstract
    Using bioinks for 3D bioprinting of cellular constructs remains a challenge due to factors including viscosity, fluid dynamics and shear stress. The encapsulation of cells within the bioinks directly affects the quality of 3D bioprinting and microfluidic pumping is a commonly used supporting approach. The accuracy of microfluidic pumps can be further improved by introducing various mixing techniques. However, many of these techniques introduce complex geometries or external fields. In this study, we used a simple control technique of time-dependent pulsing for instant gelation of the peptide bioinks and observed its effect during the bioprinting process. Various time-dependent periodic signals are imposed on to a stable flow cycle and the effects are analyzed. The microfluidic pumps are programmed with different flow patterns represented by low frequency sinusoidal pulses, ramp inputs, and duty cycle pulses. Different combinations of these pulses are tested to achieve an optimal pulse for improved quality of printed constructs. Time-varied pulsing of microfluidic pumps, particularly as square waveforms, is found to provide better continuous flow and avoid material buildup within the extruder unit when compared to pumping at a constant flow rate with manual tuning. Clogging is avoided since the gelation rate is periodically reduced which avoids gel clumps in the printed constructs. This study substantially improves the use of suitable peptide bioinks, standardizes the 3D bioprinting process, and reduces clogging and clumping during printing. Our findings allow for printing of more accurate and complex constructs for applications in tissue engineering, such as skin grafting, and other regenerative medical applications.
    Citation
    Khan, Z., Kahin, K., & Hauser, C. (2021). Time-dependent pulsing of microfluidic pumps to enhance 3D bioprinting of peptide bioinks. Microfluidics, BioMEMS, and Medical Microsystems XIX. doi:10.1117/12.2578830
    Sponsors
    The research reported in this publication was supported by funding from the King Abdullah University of Science and Technology (KAUST). We would like to thank Hamed Albalawi for his support in designing the test files in SolidWorks.
    Publisher
    SPIE-Intl Soc Optical Eng
    Conference/Event name
    Microfluidics, BioMEMS, and Medical Microsystems XIX 2020
    ISBN
    9781510641099
    DOI
    10.1117/12.2578830
    Additional Links
    https://www.spiedigitallibrary.org/conference-proceedings-of-spie/11637/2578830/Time-dependent-pulsing-of-microfluidic-pumps-to-enhance-3D-bioprinting/10.1117/12.2578830.full
    ae974a485f413a2113503eed53cd6c53
    10.1117/12.2578830
    Scopus Count
    Collections
    Conference Papers; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program; Environmental Science and Engineering Program; Computational Bioscience Research Center (CBRC)

    entitlement

     
    DSpace software copyright © 2002-2022  DuraSpace
    Quick Guide | Contact Us | KAUST University Library
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.