CD44 and PSGL-1 Collaborate in Controlling the Migration of Human Activated T-Cells
dc.contributor.author | Ali, Amal J. | |
dc.contributor.author | Merzaban, Jasmeen | |
dc.contributor.author | AbuElela, Ayman | |
dc.date.accessioned | 2021-04-11T08:52:04Z | |
dc.date.available | 2021-04-11T08:52:04Z | |
dc.date.issued | 2015-12-03 | |
dc.identifier.citation | Ali, A. J., Merzaban, J. S., & Abuelela, A. F. (2015). CD44 and PSGL-1 Collaborate in Controlling the Migration of Human Activated T-Cells. Blood, 126(23), 3429–3429. doi:10.1182/blood.v126.23.3429.3429 | |
dc.identifier.issn | 0006-4971 | |
dc.identifier.issn | 1528-0020 | |
dc.identifier.doi | 10.1182/blood.v126.23.3429.3429 | |
dc.identifier.uri | http://hdl.handle.net/10754/668637 | |
dc.description.abstract | Abstract Selectins play a central role in the trafficking of activated T cells by mediating tethering and rolling on vascular endothelium, acting as a beacon to help navigate them to the site of infection. Here we present a comprehensive binding and in vitro functional analysis of E-selectin ligands expressed on human activated T cells. Using a mass-spectrometric approach we identified a number of glycoproteins that may act as physiological E-selectin ligands on human activated T cells and focused on comparing the role the previously identified well-known E-selectin ligands, PSGL-1 (P-selectin glycoprotein ligand 1) to the newly identified ligand CD44. We show that CD44 from human activated CD4+ and CD8+ T cells binds E-selectin, and that immobilized CD44 mediates tethering and rolling of E-selectin expressing CHO cells via sialylated N-linked glycans. By knocking down CD44 and/or PSGL-1 in primary human activated T cells, our data demonstrate for the first time that CD44 is essential for mediating the rolling over E-selectin and thereby cooperates with PSGL-1 as a major E-selectin ligand on human activated T cells. This has major implications in the development of targeted therapies to combat inflammatory diseases and in transplantation settings. Disclosures No relevant conflicts of interest to declare. | |
dc.publisher | American Society of Hematology | |
dc.relation.url | https://ashpublications.org/blood/article/126/23/3429/91085/CD44-and-PSGL1-Collaborate-in-Controlling-the | |
dc.rights | Archived with thanks to Blood | |
dc.title | CD44 and PSGL-1 Collaborate in Controlling the Migration of Human Activated T-Cells | |
dc.type | Article | |
dc.contributor.department | Biological and Environmental Science and Engineering (BESE) Division | |
dc.contributor.department | Bioscience Program | |
dc.identifier.journal | Blood | |
dc.eprint.version | Post-print | |
dc.identifier.volume | 126 | |
dc.identifier.issue | 23 | |
dc.identifier.pages | 3429-3429 | |
kaust.person | Ali, Amal J. | |
kaust.person | Merzaban, Jasmeen S. | |
kaust.person | AbuElela, Ayman |
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