CD44 and PSGL-1 Collaborate in Controlling the Migration of Human Activated T-Cells
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/668637
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AbstractAbstract Selectins play a central role in the trafficking of activated T cells by mediating tethering and rolling on vascular endothelium, acting as a beacon to help navigate them to the site of infection. Here we present a comprehensive binding and in vitro functional analysis of E-selectin ligands expressed on human activated T cells. Using a mass-spectrometric approach we identified a number of glycoproteins that may act as physiological E-selectin ligands on human activated T cells and focused on comparing the role the previously identified well-known E-selectin ligands, PSGL-1 (P-selectin glycoprotein ligand 1) to the newly identified ligand CD44. We show that CD44 from human activated CD4+ and CD8+ T cells binds E-selectin, and that immobilized CD44 mediates tethering and rolling of E-selectin expressing CHO cells via sialylated N-linked glycans. By knocking down CD44 and/or PSGL-1 in primary human activated T cells, our data demonstrate for the first time that CD44 is essential for mediating the rolling over E-selectin and thereby cooperates with PSGL-1 as a major E-selectin ligand on human activated T cells. This has major implications in the development of targeted therapies to combat inflammatory diseases and in transplantation settings. Disclosures No relevant conflicts of interest to declare.
CitationAli, A. J., Merzaban, J. S., & Abuelela, A. F. (2015). CD44 and PSGL-1 Collaborate in Controlling the Migration of Human Activated T-Cells. Blood, 126(23), 3429–3429. doi:10.1182/blood.v126.23.3429.3429
PublisherAmerican Society of Hematology