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    CD44 and PSGL-1 Collaborate in Controlling the Migration of Human Activated T-Cells

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    Type
    Article
    Authors
    Ali, Amal J. cc
    Merzaban, Jasmeen cc
    AbuElela, Ayman cc
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Bioscience Program
    Date
    2015-12-03
    Permanent link to this record
    http://hdl.handle.net/10754/668637
    
    Metadata
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    Abstract
    Abstract Selectins play a central role in the trafficking of activated T cells by mediating tethering and rolling on vascular endothelium, acting as a beacon to help navigate them to the site of infection. Here we present a comprehensive binding and in vitro functional analysis of E-selectin ligands expressed on human activated T cells. Using a mass-spectrometric approach we identified a number of glycoproteins that may act as physiological E-selectin ligands on human activated T cells and focused on comparing the role the previously identified well-known E-selectin ligands, PSGL-1 (P-selectin glycoprotein ligand 1) to the newly identified ligand CD44. We show that CD44 from human activated CD4+ and CD8+ T cells binds E-selectin, and that immobilized CD44 mediates tethering and rolling of E-selectin expressing CHO cells via sialylated N-linked glycans. By knocking down CD44 and/or PSGL-1 in primary human activated T cells, our data demonstrate for the first time that CD44 is essential for mediating the rolling over E-selectin and thereby cooperates with PSGL-1 as a major E-selectin ligand on human activated T cells. This has major implications in the development of targeted therapies to combat inflammatory diseases and in transplantation settings. Disclosures No relevant conflicts of interest to declare.
    Citation
    Ali, A. J., Merzaban, J. S., & Abuelela, A. F. (2015). CD44 and PSGL-1 Collaborate in Controlling the Migration of Human Activated T-Cells. Blood, 126(23), 3429–3429. doi:10.1182/blood.v126.23.3429.3429
    Publisher
    American Society of Hematology
    Journal
    Blood
    DOI
    10.1182/blood.v126.23.3429.3429
    Additional Links
    https://ashpublications.org/blood/article/126/23/3429/91085/CD44-and-PSGL1-Collaborate-in-Controlling-the
    ae974a485f413a2113503eed53cd6c53
    10.1182/blood.v126.23.3429.3429
    Scopus Count
    Collections
    Articles; Biological and Environmental Science and Engineering (BESE) Division; Bioscience Program

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