Seasonal dynamics of natural Ostreococcus viral infection at the single cell level using VirusFISH.
dc.contributor.author | M Castillo, Yaiza | |
dc.contributor.author | Forn, Irene | |
dc.contributor.author | Yau, Sheree | |
dc.contributor.author | Moran, Xose Anxelu G. | |
dc.contributor.author | Alonso-Sáez, Laura | |
dc.contributor.author | Arandia-Gorostidi, Néstor | |
dc.contributor.author | Vaqué, Dolors | |
dc.contributor.author | Sebastián, Marta | |
dc.date.accessioned | 2021-04-07T08:25:29Z | |
dc.date.available | 2021-04-07T08:25:29Z | |
dc.date.issued | 2021-04-05 | |
dc.identifier.citation | Castillo, Y. M., Forn, I., Yau, S., Morán, X. A. G., Alonso-Sáez, L., Arandia-Gorostidi, N., … Sebastián, M. (2021). Seasonal dynamics of natural Ostreococcus viral infection at the single cell level using VirusFISH. Environmental Microbiology. doi:10.1111/1462-2920.15504 | |
dc.identifier.issn | 1462-2912 | |
dc.identifier.pmid | 33817943 | |
dc.identifier.doi | 10.1111/1462-2920.15504 | |
dc.identifier.uri | http://hdl.handle.net/10754/668586 | |
dc.description.abstract | Ostreococcus is a cosmopolitan marine genus of phytoplankton found in mesotrophic and oligotrophic waters, and the smallest free-living eukaryotes known to date, with a cell diameter close to 1 μm. Ostreococcus has been extensively studied as a model system to investigate viral-host dynamics in culture, yet the impact of viruses in naturally occurring populations is largely unknown. Here, we used Virus Fluorescence in situ Hybridization (VirusFISH) to visualize and quantify viral-host dynamics in natural populations of Ostreococcus during a seasonal cycle in the central Cantabrian Sea (Southern Bay of Biscay). Ostreococcus were predominantly found during summer and autumn at surface and 50 m depth, in coastal, mid-shelf and shelf waters, representing up to 21% of the picoeukaryotic communities. Viral infection was only detected in surface waters, and its impact was variable but highest from May to July and November to December, when up to half of the population was infected. Metatranscriptomic data available from the mid-shelf station unveiled that the Ostreococcus population was dominated by the species O. lucimarinus. This work represents a proof of concept that the VirusFISH technique can be used to quantify the impact of viruses on targeted populations of key microbes from complex natural communities. This article is protected by copyright. All rights reserved. | |
dc.description.sponsorship | This work was supported by the Spanish projects MEFISTO (CTM2013-43767-P, MINECO) and ALLFLAGS (CTM2016-75083-R, MINECO). YMC was supported by a FPI Spanish fellowship (BES-2014-067849), SY was supported by a Juan de la Cierva fellowship (IJCI-2017-34245) and LAS was supported by a Juan de la Cierva and Ramon y Cajal contract (RYC-2012-11404). MS was supported by a Viera y Clavijo contract funded by the ACIISI and the ULPGC. NAG was supported by Basque Government’s PhD-program fellowship. The authors declare that there is no conflict of interest regarding the publication of this article. | |
dc.publisher | Wiley | |
dc.relation.url | https://onlinelibrary.wiley.com/doi/10.1111/1462-2920.15504 | |
dc.rights | Archived with thanks to Environmental microbiology | |
dc.title | Seasonal dynamics of natural Ostreococcus viral infection at the single cell level using VirusFISH. | |
dc.type | Article | |
dc.contributor.department | Biological and Environmental Sciences and Engineering (BESE) Division | |
dc.contributor.department | Marine Science Program | |
dc.contributor.department | Red Sea Research Center (RSRC) | |
dc.identifier.journal | Environmental microbiology | |
dc.rights.embargodate | 2022-04-05 | |
dc.eprint.version | Post-print | |
dc.contributor.institution | Department of Marine Biology and Oceanography Institute of Marine Sciences (CSIC) Barcelona Spain | |
dc.contributor.institution | Centro Oceanográfico de Gijón/Xixón, IEO Gijón Xixón Spain | |
dc.contributor.institution | AZTI Marine Research, Basque Research and Technology Alliance (BRTA), Txatxarramendi ugartea z/g Sukarrieta Spain | |
dc.contributor.institution | Department of Earth System Science Stanford University Stanford CA USA | |
dc.contributor.institution | Institute of Oceanography and Global Change (IOCAG) University of Las Palmas de Gran Canaria (ULPGC) Telde Spain | |
kaust.person | Moran, Xose Anxelu G. | |
refterms.dateFOA | 2021-04-07T08:26:30Z |
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