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dc.contributor.authorM Castillo, Yaiza
dc.contributor.authorForn, Irene
dc.contributor.authorYau, Sheree
dc.contributor.authorMoran, Xose Anxelu G.
dc.contributor.authorAlonso-Sáez, Laura
dc.contributor.authorArandia-Gorostidi, Néstor
dc.contributor.authorVaqué, Dolors
dc.contributor.authorSebastián, Marta
dc.date.accessioned2021-04-07T08:25:29Z
dc.date.available2021-04-07T08:25:29Z
dc.date.issued2021-04-05
dc.identifier.citationCastillo, Y. M., Forn, I., Yau, S., Morán, X. A. G., Alonso-Sáez, L., Arandia-Gorostidi, N., … Sebastián, M. (2021). Seasonal dynamics of natural Ostreococcus viral infection at the single cell level using VirusFISH. Environmental Microbiology. doi:10.1111/1462-2920.15504
dc.identifier.issn1462-2912
dc.identifier.pmid33817943
dc.identifier.doi10.1111/1462-2920.15504
dc.identifier.urihttp://hdl.handle.net/10754/668586
dc.description.abstractOstreococcus is a cosmopolitan marine genus of phytoplankton found in mesotrophic and oligotrophic waters, and the smallest free-living eukaryotes known to date, with a cell diameter close to 1 μm. Ostreococcus has been extensively studied as a model system to investigate viral-host dynamics in culture, yet the impact of viruses in naturally occurring populations is largely unknown. Here, we used Virus Fluorescence in situ Hybridization (VirusFISH) to visualize and quantify viral-host dynamics in natural populations of Ostreococcus during a seasonal cycle in the central Cantabrian Sea (Southern Bay of Biscay). Ostreococcus were predominantly found during summer and autumn at surface and 50 m depth, in coastal, mid-shelf and shelf waters, representing up to 21% of the picoeukaryotic communities. Viral infection was only detected in surface waters, and its impact was variable but highest from May to July and November to December, when up to half of the population was infected. Metatranscriptomic data available from the mid-shelf station unveiled that the Ostreococcus population was dominated by the species O. lucimarinus. This work represents a proof of concept that the VirusFISH technique can be used to quantify the impact of viruses on targeted populations of key microbes from complex natural communities. This article is protected by copyright. All rights reserved.
dc.description.sponsorshipThis work was supported by the Spanish projects MEFISTO (CTM2013-43767-P, MINECO) and ALLFLAGS (CTM2016-75083-R, MINECO). YMC was supported by a FPI Spanish fellowship (BES-2014-067849), SY was supported by a Juan de la Cierva fellowship (IJCI-2017-34245) and LAS was supported by a Juan de la Cierva and Ramon y Cajal contract (RYC-2012-11404). MS was supported by a Viera y Clavijo contract funded by the ACIISI and the ULPGC. NAG was supported by Basque Government’s PhD-program fellowship. The authors declare that there is no conflict of interest regarding the publication of this article.
dc.publisherWiley
dc.relation.urlhttps://onlinelibrary.wiley.com/doi/10.1111/1462-2920.15504
dc.rightsArchived with thanks to Environmental microbiology
dc.titleSeasonal dynamics of natural Ostreococcus viral infection at the single cell level using VirusFISH.
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentMarine Science Program
dc.contributor.departmentMicrobial oceanography Research Group
dc.contributor.departmentRed Sea Research Center (RSRC)
dc.identifier.journalEnvironmental microbiology
dc.rights.embargodate2022-04-05
dc.eprint.versionPost-print
dc.contributor.institutionDepartment of Marine Biology and Oceanography Institute of Marine Sciences (CSIC) Barcelona Spain
dc.contributor.institutionCentro Oceanográfico de Gijón/Xixón, IEO Gijón Xixón Spain
dc.contributor.institutionAZTI Marine Research, Basque Research and Technology Alliance (BRTA), Txatxarramendi ugartea z/g Sukarrieta Spain
dc.contributor.institutionDepartment of Earth System Science Stanford University Stanford CA USA
dc.contributor.institutionInstitute of Oceanography and Global Change (IOCAG) University of Las Palmas de Gran Canaria (ULPGC) Telde Spain
kaust.personMoran, Xose Anxelu G.
refterms.dateFOA2021-04-07T08:26:30Z


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