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    Frequency-modulation Stimulated Raman Scattering microscopy with an Acousto-Optic Tunable Filter

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    Name:
    MS Thesis_Elisa Grassi_Final.pdf
    Size:
    11.29Mb
    Format:
    PDF
    Description:
    MS Thesis
    Embargo End Date:
    2022-10-06
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    Type
    Thesis
    Authors
    Grassi, Elisa cc
    Advisors
    Liberale, Carlo cc
    Committee members
    Habuchi, Satoshi cc
    Hauser, Charlotte cc
    Program
    Bioengineering
    KAUST Department
    Biological and Environmental Science and Engineering (BESE) Division
    Date
    2021-04
    Embargo End Date
    2022-10-06
    Permanent link to this record
    http://hdl.handle.net/10754/668583
    
    Metadata
    Show full item record
    Access Restrictions
    At the time of archiving, the student author of this thesis opted to temporarily restrict access to it. The full text of this thesis will become available to the public after the expiration of the embargo on 2022-10-06.
    Abstract
    Stimulated Raman Scattering (SRS) is a Coherent Raman microscopy method that has been increasingly employed in recent years for highly-specific, label-free, and high-speed bioimaging. Compared to a similar Coherent Raman method, the so-called Coherent Anti-Stokes Scattering (CARS) microscopy, it exhibits advantages such as the absence of nonresonant background (NRB) and the linearity of the signal intensity on the concentration of molecules of interest. However, SRS can be affected by unwanted background signals that hinder the acquisition of an accurate Raman information. These unwanted signals are generated by parasitic effects that are difficult to suppress in standard SRS setups. Here, I present a frequency-modulation (FM) SRS technique via an Acousto-Optic Tunable ioilter (AOTF), describing its implementation on Vibra Lab setup and assessing its efficiency with imaging results. The FM technique provides a cancellation of the unwanted background signals, maintaining intact the SRS information. It is based on the weak spectral dependence of the parasitic effects as compared to the high spectral specificity of the SRS signal. The proposed scheme presents a few advantages when compared with other solutions presented in the literature. In particular, it doesn't require a complex setup configuration, and it can be used seamlessly in a very broad range of the vibrational spectrum.
    Citation
    Grassi, E. (2021). Frequency-modulation Stimulated Raman Scattering microscopy with an Acousto-Optic Tunable Filter. KAUST Research Repository. https://doi.org/10.25781/KAUST-0476R
    DOI
    10.25781/KAUST-0476R
    ae974a485f413a2113503eed53cd6c53
    10.25781/KAUST-0476R
    Scopus Count
    Collections
    Bioengineering Program; Biological and Environmental Science and Engineering (BESE) Division; MS Theses

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