Show simple item record

dc.contributor.authorAhmed, Muhammad Saad
dc.contributor.authorLauersen, Kyle J.
dc.contributor.authorIkram, Sana
dc.contributor.authorLi, Chun
dc.date.accessioned2021-03-24T07:55:35Z
dc.date.available2021-03-24T07:55:35Z
dc.date.issued2021-03-22
dc.date.submitted2020-10-07
dc.identifier.citationAhmed, M. S., Lauersen, K. J., Ikram, S., & Li, C. (2021). Efflux Transporters’ Engineering and Their Application in Microbial Production of Heterologous Metabolites. ACS Synthetic Biology. doi:10.1021/acssynbio.0c00507
dc.identifier.issn2161-5063
dc.identifier.issn2161-5063
dc.identifier.pmid33751883
dc.identifier.doi10.1021/acssynbio.0c00507
dc.identifier.urihttp://hdl.handle.net/10754/668230
dc.description.abstractMetabolic engineering of microbial hosts for the production of heterologous metabolites and biochemicals is an enabling technology to generate meaningful quantities of desired products that may be otherwise difficult to produce by traditional means. Heterologous metabolite production can be restricted by the accumulation of toxic products within the cell. Efflux transport proteins (transporters) provide a potential solution to facilitate the export of these products, mitigate toxic effects, and enhance production. Recent investigations using knockout lines, heterologous expression, and expression profiling of transporters have revealed candidates that can enhance the export of heterologous metabolites from microbial cell systems. Transporter engineering efforts have revealed that some exhibit flexible substrate specificity and may have broader application potentials. In this Review, the major superfamilies of efflux transporters, their mechanistic modes of action, selection of appropriate efflux transporters for desired compounds, and potential transporter engineering strategies are described for potential applications in enhancing engineered microbial metabolite production. Future studies in substrate recognition, heterologous expression, and combinatorial engineering of efflux transporters will assist efforts to enhance heterologous metabolite production in microbial hosts.
dc.description.sponsorshipWe are highly grateful to Mr. Iordachescu Ilie Mihaita Bogdan for assisting in reviewing the Review.
dc.publisherAmerican Chemical Society (ACS)
dc.relation.urlhttps://pubs.acs.org/doi/10.1021/acssynbio.0c00507
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Synthetic Biology, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acssynbio.0c00507.
dc.titleEfflux Transporters’ Engineering and Their Application in Microbial Production of Heterologous Metabolites
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentBiological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955, Kingdom of Saudi Arabia
dc.identifier.journalACS Synthetic Biology
dc.rights.embargodate2022-03-22
dc.eprint.versionPost-print
dc.contributor.institutionInstitute for Synthetic Biosystem/Department of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology (BIT), Beijing 100081, P. R. China
dc.contributor.institutionDepartment of Biological Sciences, National University of Medical Sciences (NUMS), Abid Majeed Road, The Mall, Rawalpindi 46000, Pakistan
dc.contributor.institutionBeijing Higher Institution Engineering Research Center for Food Additives and Ingredients, Beijing Technology & Business University (BTBU), Beijing 100048, P. R. China
dc.contributor.institutionSynBio Research Platform, Collaborative Innovation Center of Chemical Science and Engineering, Key Laboratory of Systems Bioengineering, Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300350, P. R. China
dc.contributor.institutionKey Laboratory for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing 100084, P. R. China
kaust.personLauersen, Kyle J.
dc.date.accepted2021-03-22
refterms.dateFOA2021-03-24T11:29:01Z
dc.date.published-online2021-03-22
dc.date.published-print2021-04-16


Files in this item

Thumbnail
Name:
Manuscript, Muhammad March 08, 2021 Final_KAUST.pdf
Size:
10.33Mb
Format:
PDF
Description:
Accepted manuscript

This item appears in the following Collection(s)

Show simple item record