Efflux Transporters’ Engineering and Their Application in Microbial Production of Heterologous Metabolites
KAUST DepartmentBiological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal 23955, Kingdom of Saudi Arabia
Online Publication Date2021-03-22
Print Publication Date2021-04-16
Embargo End Date2022-03-22
Permanent link to this recordhttp://hdl.handle.net/10754/668230
MetadataShow full item record
AbstractMetabolic engineering of microbial hosts for the production of heterologous metabolites and biochemicals is an enabling technology to generate meaningful quantities of desired products that may be otherwise difficult to produce by traditional means. Heterologous metabolite production can be restricted by the accumulation of toxic products within the cell. Efflux transport proteins (transporters) provide a potential solution to facilitate the export of these products, mitigate toxic effects, and enhance production. Recent investigations using knockout lines, heterologous expression, and expression profiling of transporters have revealed candidates that can enhance the export of heterologous metabolites from microbial cell systems. Transporter engineering efforts have revealed that some exhibit flexible substrate specificity and may have broader application potentials. In this Review, the major superfamilies of efflux transporters, their mechanistic modes of action, selection of appropriate efflux transporters for desired compounds, and potential transporter engineering strategies are described for potential applications in enhancing engineered microbial metabolite production. Future studies in substrate recognition, heterologous expression, and combinatorial engineering of efflux transporters will assist efforts to enhance heterologous metabolite production in microbial hosts.
CitationAhmed, M. S., Lauersen, K. J., Ikram, S., & Li, C. (2021). Efflux Transporters’ Engineering and Their Application in Microbial Production of Heterologous Metabolites. ACS Synthetic Biology. doi:10.1021/acssynbio.0c00507
SponsorsWe are highly grateful to Mr. Iordachescu Ilie Mihaita Bogdan for assisting in reviewing the Review.
PublisherAmerican Chemical Society (ACS)
JournalACS Synthetic Biology
- Getting pumped: membrane efflux transporters for enhanced biomolecule production.
- Authors: Boyarskiy S, Tullman-Ercek D
- Issue date: 2015 Oct
- Efflux transporter engineering markedly improves amorphadiene production in Escherichia coli.
- Authors: Zhang C, Chen X, Stephanopoulos G, Too HP
- Issue date: 2016 Aug
- Improving heterologous polyketide production in Escherichia coli by transporter engineering.
- Authors: Yang J, Xiong ZQ, Song SJ, Wang JF, Lv HJ, Wang Y
- Issue date: 2015 Oct
- Efflux systems in bacteria and their metabolic engineering applications.
- Authors: Jones CM, Hernández Lozada NJ, Pfleger BF
- Issue date: 2015 Nov
- Engineering transport systems for microbial production.
- Authors: Onyeabor M, Martinez R, Kurgan G, Wang X
- Issue date: 2020