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dc.contributor.authorPinel, I.S.M.
dc.contributor.authorHankinson, P.M.
dc.contributor.authorMoed, D.H.
dc.contributor.authorWyseure, L.J.
dc.contributor.authorVrouwenvelder, Johannes S.
dc.contributor.authorvan Loosdrecht, Mark C.M.
dc.date.accessioned2021-03-21T12:56:45Z
dc.date.available2021-03-21T12:56:45Z
dc.date.issued2021-03-15
dc.date.submitted2020-08-12
dc.identifier.citationPinel, I. S. M., Hankinson, P. M., Moed, D. H., Wyseure, L. J., Vrouwenvelder, J. S., & van Loosdrecht, M. C. M. (2021). Efficient cooling tower operation at alkaline pH for the control of Legionella pneumophila and other pathogenic genera. Water Research, 117047. doi:10.1016/j.watres.2021.117047
dc.identifier.issn0043-1354
dc.identifier.doi10.1016/j.watres.2021.117047
dc.identifier.urihttp://hdl.handle.net/10754/668167
dc.description.abstractEfficient control of pathogenic bacteria, specifically Legionella pneumophila, is one of the main concerns when operating industrial cooling towers. Common practices to limit proliferation involves use of disinfectants, leading to formation of disinfection by-product and increase in water corrosiveness. A disinfectant-free Legionella control method would make the industry more environmentally friendly. A pilot-scale cooling tower (1 m3/h) operated with demineralized water was used to investigate the potential of high-pH conditioning as a disinfectant-free alternative for control of L. pneumophila and other pathogens. One control experiment was performed under standard full-scale operation involving sodium hypochlorite dosage. Thereafter 3 alkaline pHs of the cooling water were tested: 9.0, 9.4 and 9.6. The tests lasted between 25 and 35 days. The cooling water from the basins were analysed for total cell count by flow cytometry, L. pneumophila concentration by plate count and occasional qPCR analyses targeting the mip-gene, bacterial and eukaryotic community analyses with 16S and 18S rRNA gene amplicon sequencing, relative abundance of eukaryotic to prokaryotic DNA by qPCR of the 16S and 18S rRNA gene. The L. pneumophila analyses showed considerable growth at pH 9.0 and pH 9.4 but was maintained below detection limit (< 100 CFU/L) at pH 9.6 without disinfection. Interestingly, the results correlated with the overall abundance of protozoa in the water samples but not directly with the relative abundance of specific reported protozoan hosts of Legionella. The pathogenicity based on 16S rRNA gene amplicon sequencing of the cooling water DNA decreased with increasing pH with a strong decline between pH 9.0 and pH 9.4, from 7.1 % to 1.6 % of relative abundance of pathogenic genera respectively. A strong shift in microbiome was observed between each tested pH and reproducibility of the experiment at pH 9.6 was confirmed with a duplicate test lasting 80 days. High-pH conditioning ≥ 9.6 is therefore considered as an efficient disinfectant-free cooling tower operation for control of pathogenicity, including L. pneumophila.
dc.description.sponsorshipThis study was performed with financial support from Evides Industriewater B.V. and with the use of their pilot facility. We would also like to thank the staff members of Aqualab Zuid B.V. for performing the L. pneumophila analyses and David Calderón Franco for his help with the 16S and 18S genes qPCR analyses.
dc.publisherElsevier BV
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S0043135421002451
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Water Research. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Water Research, [, , (2021-03-15)] DOI: 10.1016/j.watres.2021.117047 . © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleEfficient cooling tower operation at alkaline pH for the control of Legionella pneumophila and other pathogenic genera
dc.typeArticle
dc.contributor.departmentEnvironmental Science and Engineering Program
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalWater Research
dc.rights.embargodate2023-03-15
dc.eprint.versionPost-print
dc.contributor.institutionDelft University of Technology, Faculty of Applied Sciences, Department of Biotechnology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.
dc.contributor.institutionEvides Industriewater, Schaardijk 150, 3063 NH Rotterdam, The Netherlands.
dc.identifier.pages117047
kaust.personVrouwenvelder, Johannes S.
dc.date.accepted2021-03-11
refterms.dateFOA2021-03-21T12:59:03Z


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