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dc.contributor.authorTashkandi, Manal
dc.contributor.authorAli, Zahir
dc.contributor.authorAljedaani, Fatimah R.
dc.contributor.authorShami, Ashwag
dc.contributor.authorMahfouz, Magdy M.
dc.date.accessioned2021-03-17T12:10:28Z
dc.date.available2021-03-17T12:10:28Z
dc.date.issued2018
dc.identifier.citationTashkandi, M., Ali, Z., Aljedaani, F., Ashwag Shami, & Magdy M. Mahfouz. (2018). Engineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato. Figshare. https://doi.org/10.6084/M9.FIGSHARE.7172468
dc.identifier.doi10.6084/m9.figshare.7172468
dc.identifier.urihttp://hdl.handle.net/10754/668116
dc.description.abstractCRISPR/Cas systems confer molecular immunity against phages and conjugative plasmids in prokaryotes. Recently, CRISPR/Cas9 systems have been used to confer interference against eukaryotic viruses. Here, we engineered $\textit{Nicotiana benthamiana}$ and tomato ($\textit{Solanum lycopersicum}$) plants with the CRISPR/Cas9 system to confer immunity against the $\textit{Tomato yellow leaf curl virus}$ (TYLCV). Targeting the TYLCV genome with Cas9-single guide RNA at the sequences encoding the coat protein (CP) or replicase (Rep) resulted in efficient virus interference, as evidenced by low accumulation of the TYLCV DNA genome in the transgenic plants. The CRISPR/Cas9-based immunity remained active across multiple generations in the $\textit{N. benthamiana}$ and tomato plants. Together, our results confirmed the efficiency of the CRISPR/Cas9 system for stable engineering of TYLCV resistance in $\textit{N. benthamiana}$ and tomato, and opens the possibilities of engineering virus resistance against single and multiple infectious viruses in other crops.
dc.publisherFigshare
dc.relation.urlhttps://tandf.figshare.com/articles/Engineering_resistance_against_i_Tomato_yellow_leaf_curl_virus_i_via_the_CRISPR_Cas9_system_in_tomato/7172468
dc.subjectSpace Science
dc.subjectMicrobiology
dc.subjectGenetics
dc.subjectBiotechnology
dc.subjectEvolutionary Biology
dc.subjectEcology
dc.subjectImmunology
dc.subjectPlant Biology
dc.subject60506 Virology
dc.titleEngineering resistance against $\textit{Tomato yellow leaf curl virus}$ via the CRISPR/Cas9 system in tomato
dc.typeDataset
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentLaboratory for Genome Engineering
dc.contributor.departmentPlant Science
dc.contributor.institutionCollege of Science, Biology Department, Kingdom of Saudi Arabia (KSA), Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia
kaust.personTashkandi, Manal
kaust.personAli, Zahir
kaust.personAljedaani, Fatimah R.
kaust.personShami, Ashwag
kaust.personMahfouz, Magdy M.
dc.relation.issupplementtoDOI:10.1080/15592324.2018.1525996
display.relations<b>Is Supplement To:</b><br/> <ul><li><i>[Article]</i> <br/> Tashkandi M, Ali Z, Aljedaani F, Shami A, Mahfouz MM (2018) Engineering resistance against Tomato yellow leaf curl virus via the CRISPR/Cas9 system in tomato. Plant Signaling & Behavior 13: e1525996. Available: http://dx.doi.org/10.1080/15592324.2018.1525996.. DOI: <a href="https://doi.org/10.1080/15592324.2018.1525996" >10.1080/15592324.2018.1525996</a> Handle: <a href="http://hdl.handle.net/10754/626438" >10754/626438</a></a></li></ul>


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