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dc.contributor.authorSheikh, Arsheed Hussain
dc.contributor.authorFraz Hussain, Rana M.
dc.contributor.authorTabassum, Naheed
dc.contributor.authorBadmi, Raghuram
dc.contributor.authorMarillonnet, Sylvestre
dc.contributor.authorScheel, Dierk
dc.contributor.authorLee, Justin
dc.contributor.authorSinha, Alok
dc.date.accessioned2021-03-10T05:43:36Z
dc.date.available2021-03-10T05:43:36Z
dc.date.issued2021-02-10
dc.date.submitted2020-11-25
dc.identifier.citationSheikh, A. H., Fraz Hussain, R. M., Tabassum, N., Badmi, R., Marillonnet, S., Scheel, D., … Sinha, A. (2021). Possible role of WRKY transcription factors in regulating immunity in Oryza sativa ssp. indica. Physiological and Molecular Plant Pathology, 114, 101623. doi:10.1016/j.pmpp.2021.101623
dc.identifier.issn1096-1178
dc.identifier.issn0885-5765
dc.identifier.doi10.1016/j.pmpp.2021.101623
dc.identifier.urihttp://hdl.handle.net/10754/668011
dc.description.abstractPlants have developed a robust transcription machinery to combat potential pathogenic organisms. One of the hallmarks of early immune responses is the activation of the WRKY transcription factors post infection. Specific WRKYs proteins from Arabidopsis are known substrates of MAPK pathway to mediate the flg22 elicited early immunity. In the current study, using the Golden Gate cloning strategy, we aim to clone the entire WRKY transcription factor family from Oryza sativa ssp. indica consisting of more than 100 members and study their MAPK interaction and subsequent role in PTI. Using a reporter LUC assay in protoplasts we investigated the early defense responses in a few interesting OsWRKY candidates. Interestingly, we observed stringent regulation of WRKY expression in cells and their transcriptional expression only under specific stress responses. The phenomenon of gene expression regulation by intron retention (IR) was prevalently observed in rice WRKY transcripts. We could show the role of WRKY8, 24, and 77 in early defense responses. It was observed that WRKY24 enhanced the expression of early defense response marker genes like NHL10 while WRKY8 and WRKY77 supressed their expression. This study highlights the complicated mechanism by which OsWRKYs expression is possibly regulated and the distinctive roles of some individual members in plant immunity. At the same time this study serves as a cautionary warning for plant researchers to be mindful of the intron retention mechanism while cloning OsWRKYs.
dc.description.sponsorshipAHS thanks NIPGR core grant and DAAD Fellowship (A/11/75070) for funding.
dc.publisherElsevier BV
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S0885576521000242
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Physiological and Molecular Plant Pathology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Physiological and Molecular Plant Pathology, [114, , (2021-02-10)] DOI: 10.1016/j.pmpp.2021.101623 . © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titlePossible role of WRKY transcription factors in regulating immunity in Oryza sativa ssp. indica
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.identifier.journalPhysiological and Molecular Plant Pathology
dc.rights.embargodate2023-02-10
dc.eprint.versionPost-print
dc.contributor.institutionSchool of Life Sciences, University of Warwick, Coventry, CV4 7AL, United Kingdom
dc.contributor.institutionDepartment of Forest Genetics and Plant Physiology, Umea Plant Science Centre (UPSC), Sweden
dc.contributor.institutionLeibniz Institute of Plant Biochemistry, Weinberg 3, Halle Saale, D-06120, Germany
dc.contributor.institutionNational Institute of Plant Genome Research, Aruna Asaf Ali Marg, New Delhi, 110065, India
dc.identifier.volume114
dc.identifier.pages101623
kaust.personSheikh, Arsheed Hussain
kaust.personTabassum, Naheed
dc.date.accepted2021-02-03
dc.identifier.eid2-s2.0-85101614401
refterms.dateFOA2021-03-10T10:43:56Z


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