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dc.contributor.authorPunkkinen, Matleena
dc.contributor.authorMahfouz, Magdy M.
dc.contributor.authorFujii, Hiroaki
dc.date.accessioned2021-03-09T06:17:56Z
dc.date.available2021-03-09T06:17:56Z
dc.date.issued2021-03-08
dc.date.submitted2020-12-03
dc.identifier.citationPunkkinen, M., Mahfouz, M. M., & Fujii, H. (2021). Chemical activation of Arabidopsis SnRK2.6 by pladienolide B. Plant Signaling & Behavior, 1885165. doi:10.1080/15592324.2021.1885165
dc.identifier.issn1559-2324
dc.identifier.doi10.1080/15592324.2021.1885165
dc.identifier.urihttp://hdl.handle.net/10754/667984
dc.description.abstractAbscisic acid (ABA) is an important phytohormone mediating osmotic stress responses. SUCROSE NONFERMENTING 1 (SNF1)-RELATED PROTEIN KINASE 2.6 (SnRK2.6, also named OPEN STOMATA1 and SNF1-RELATED KINASE 2E) is central in the ABA signaling pathway; therefore, manipulating its activity may be useful to confer stress tolerance in plants. Pladienolide B (PB) is an mRNA splicing inhibitor and enhances ABA responses. Here, we analyzed the effect of PB on Arabidopsis SnRK2.6. PB enhanced the activity of recombinant SnRK2.6 in vitro through direct physical interaction as predicted by molecular docking simulations followed by mutation experiments and isothermal titration calorimetry. Structural modeling predicted probable interaction sites between PB and SnRK2.6, and experiments with mutated SnRK2.6 revealed that Leu-46 was the most essential amino acid residue for SnRK2.6 activation by PB. This study demonstrates the feasibility of SnRK2.6 chemical manipulation and paves the way for the modification of plant osmotic stress responses.
dc.description.sponsorshipThis work was supported by Turku Collegium for Science and Medicine and by the Academy of Finland under Projects number [259169, 263853, 271832, 292763, 307335 to HF].
dc.description.sponsorshipWe thank Dr. Jarmo Käpylä for technical advice on the ITC measurements.
dc.publisherInforma UK Limited
dc.relation.urlhttps://www.tandfonline.com/doi/full/10.1080/15592324.2021.1885165
dc.rightsArchived with thanks to Plant Signaling & Behavior
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleChemical activation of Arabidopsis SnRK2.6 by pladienolide B
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentDesert Agriculture Initiative
dc.contributor.departmentLaboratory for Genome Engineering
dc.contributor.departmentPlant Science
dc.identifier.journalPlant Signaling & Behavior
dc.rights.embargodate2022-03-08
dc.eprint.versionPost-print
dc.contributor.institutionMolecular Plant Biology Unit, Department of Biochemistry, University of Turku, Turku, Finland
dc.contributor.institutionDepartment of Life Technologies, University of Turku, Turku,Finland
dc.identifier.pages1885165
kaust.personMahfouz, Magdy M.
dc.date.accepted2021-01-30


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