KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Desert Agriculture Initiative
Laboratory for Genome Engineering
Embargo End Date2022-03-08
Permanent link to this recordhttp://hdl.handle.net/10754/667984
MetadataShow full item record
AbstractAbscisic acid (ABA) is an important phytohormone mediating osmotic stress responses. SUCROSE NONFERMENTING 1 (SNF1)-RELATED PROTEIN KINASE 2.6 (SnRK2.6, also named OPEN STOMATA1 and SNF1-RELATED KINASE 2E) is central in the ABA signaling pathway; therefore, manipulating its activity may be useful to confer stress tolerance in plants. Pladienolide B (PB) is an mRNA splicing inhibitor and enhances ABA responses. Here, we analyzed the effect of PB on Arabidopsis SnRK2.6. PB enhanced the activity of recombinant SnRK2.6 in vitro through direct physical interaction as predicted by molecular docking simulations followed by mutation experiments and isothermal titration calorimetry. Structural modeling predicted probable interaction sites between PB and SnRK2.6, and experiments with mutated SnRK2.6 revealed that Leu-46 was the most essential amino acid residue for SnRK2.6 activation by PB. This study demonstrates the feasibility of SnRK2.6 chemical manipulation and paves the way for the modification of plant osmotic stress responses.
CitationPunkkinen, M., Mahfouz, M. M., & Fujii, H. (2021). Chemical activation of Arabidopsis SnRK2.6 by pladienolide B. Plant Signaling & Behavior, 1885165. doi:10.1080/15592324.2021.1885165
SponsorsThis work was supported by Turku Collegium for Science and Medicine and by the Academy of Finland under Projects number [259169, 263853, 271832, 292763, 307335 to HF].
We thank Dr. Jarmo Käpylä for technical advice on the ITC measurements.
PublisherInforma UK Limited
JournalPlant Signaling & Behavior
Except where otherwise noted, this item's license is described as Archived with thanks to Plant Signaling & Behavior