Astrocytes and learning dependent synaptic stabilization: Role of glycogen-derived lactate
Type
PosterAuthors
Vezzoli, ElenaCali, Corrado
Ponzoni, Luisa
Sogne, Elisa

Gagnon, Nicolas
Francolini, Maura
Braida, Daniela
Sala, Mariaelvina
Falqui, Andrea

Magistretti, Pierre J.

KAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionBioscience Program
King Abdullah University of Science and Technology (KAUST), Biological and Environmental Science & Engineering (BESE) Division, Milan, Italy
Date
2019-09Permanent link to this record
http://hdl.handle.net/10754/667976
Metadata
Show full item recordAbstract
Long-term memory formation is a process accompanied by energy-expensive structural changes at synapses, such as increased spine density. Besides, parallel increases in both spine volume and postsynaptic density surface have been suggested but never quantified in vivo by clear-cut experimental evidences. To directly investigate structural changes occurring during learning, and their dependence on brain energy metabolism, an in-depth 3D Electron Microscopy (EM) study was performed on adult mice brains subjected to a novel-object recognition (NOR) behavioral training, in presence of 1,4-dideoxy-1,4-imino-D-arabinitol hydrochloride (DAB), a potent inhibitor of glycogenolysis. Memory consolidation impairment induced by the DAB treatment was reversed by intrahippocampal injection of L-lactate. The following 3D ultrastructural analysis on sparse reconstruction of spines and synaptic densities revealed that both density and size of spines increased significantly compared to naive animals, together with the appearance of glycogen clusters in astrocyte processes. The DAB treatment impaired the formation of new spines, and the application of L-lactate together with the DAB rescued both memory formation and spine density, but failed to rescue the accumulation of glycogen clusters. Moreover, 3D analyses of dendritic mitochondria revealed an impaired fission, which was also rescued by intrahippocampal L-lactate administration. All these results suggest that the energy and signaling provided by glycogen-derived L-lactate is necessary for the structural synaptic changes related to memory consolidation.Citation
Vezzoli, E., Calì, C., Ponzoni, L., Sogne, E., Gagnon, N., Francolini, M., … Magistretti, P. J. (2019). Astrocytes and learning dependent synaptic stabilization: Role of glycogen-derived lactate. IBRO Reports, 6, S381. doi:10.1016/j.ibror.2019.07.1211Publisher
Elsevier BVConference/Event name
The 10th IBRO World Congress of NeuroscienceAdditional Links
https://linkinghub.elsevier.com/retrieve/pii/S2451830119312634ae974a485f413a2113503eed53cd6c53
10.1016/j.ibror.2019.07.1211