Gut microbiota modulates genes involved in the astrocyte-neuron lactate shuttle in the hippocampus
AuthorsMargineanu, Michael B.
Cryan, John F.
Magistretti, Pierre J.
KAUST DepartmentBioscience Program
Biological and Environmental Sciences and Engineering (BESE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/667975
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AbstractThe gut microbiota modulates brain physiology, development, and behavior and has been implicated as a key regulator in several central nervous system disorders. Its effect on the metabolic coupling between neurons and astrocytes has not been extensively studied to date, even though this is an important component of brain energy metabolism and physiology and it is perturbed in neurodegenerative and cognitive disorders. In this study, we have investigated the mRNA expression of 6 genes encoding proteins implicated in the Astrocyte-Neuron Lactate Shuttle (Atp1a2, Ldha, Ldhb, Mct1, Gys1, Pfkfb3), in relation to different gut microbiota manipulations, in the mouse brain hippocampus, a region with critical functions in cognition and behavior. We have discovered that Atp1a2 and Pfkfb3, encoding the ATPase, Na+/K+ transporting, alpha 2 sub-unit, and respectively 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3, two genes predominantly expressed in astrocytes, were upregulated in the hippocampus of germ-free mice in the absence of a microbiota and also after limited re-colonization of microbiota for 24 h, compared to conventionally raised mice. The increase in mRNA expression of Atp1a2 was also confirmed on the protein level. Atp1a2 and Pfkfb3 were also upregulated upon diet supplementation with prebiotics (fructo- and galacto-oligosaccharides) in the hippocampus of mice that underwent a protocol inducing chronic psychosocial stress. To our knowledge, these findings are the first to report an influence of the gut microbiota and prebiotics on mRNA expression of genes implicated in the metabolic coupling between neurons and astrocytes.
CitationMargineanu, M., Sherwin, E., Golubeva, A., Peterson, V., Hoban, A., Rea, K., … Magistretti, P. (2019). Gut microbiota modulates genes involved in the astrocyte-neuron lactate shuttle in the hippocampus. IBRO Reports, 6, S379–S380. doi:10.1016/j.ibror.2019.07.1206
Conference/Event nameThe 10th IBRO World Congress of Neuroscience