Fine-scale metabolic discontinuity in a stratified prokaryote microbiome of a Red Sea deep halocline
Type
ArticleAuthors
Michoud, Gregoire
Ngugi, David

Barozzi, Alan

Merlino, Giuseppe

Calleja Cortes, Maria de Lluch
Delgado-Huertas, Antonio

Moran, Xose Anxelu G.

Daffonchio, Daniele

KAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionBioscience Program
Extreme Systems Microbiology Lab
Marine Science Program
Microbial oceanography Research Group
Red Sea Research Center (RSRC)
Date
2021-03-01Online Publication Date
2021-03-01Print Publication Date
2021-08Embargo End Date
2021-09-01Submitted Date
2020-07-04Permanent link to this record
http://hdl.handle.net/10754/667877
Metadata
Show full item recordAbstract
AbstractDeep-sea hypersaline anoxic basins are polyextreme environments in the ocean’s interior characterized by the high density of brines that prevents mixing with the overlaying seawater, generating sharp chemoclines and redoxclines up to tens of meters thick that host a high concentration of microbial communities. Yet, a fundamental understanding of how such pycnoclines shape microbial life and the associated biogeochemical processes at a fine scale, remains elusive. Here, we applied high-precision sampling of the brine–seawater transition interface in the Suakin Deep, located at 2770 m in the central Red Sea, to reveal previously undocumented fine-scale community structuring and succession of metabolic groups along a salinity gradient only 1 m thick. Metagenomic profiling at a 10-cm-scale resolution highlighted spatial organization of key metabolic pathways and corresponding microbial functional units, emphasizing the prominent role and significance of salinity and oxygen in shaping their ecology. Nitrogen cycling processes are especially affected by the redoxcline with ammonia oxidation processes being taxa and layers specific, highlighting also the presence of novel microorganisms, such as novel Thaumarchaeota and anammox, adapted to the changing conditions of the chemocline. The findings render the transition zone as a critical niche for nitrogen cycling, with complementary metabolic networks, in turn underscoring the biogeochemical complexity of deep-sea brines.Citation
Michoud, G., Ngugi, D. K., Barozzi, A., Merlino, G., Calleja, M. L., Delgado-Huertas, A., … Daffonchio, D. (2021). Fine-scale metabolic discontinuity in a stratified prokaryote microbiome of a Red Sea deep halocline. The ISME Journal. doi:10.1038/s41396-021-00931-zSponsors
This research was supported by King Abdullah University of Science and Technology through baseline funding and Red Sea Research Center competitive fund to DD. We thank the crew of R/V Thuwal and CMOR for their support in sampling the brines, especially Brian Hession and Thomas Hoover. We also thank personal of the KAUST Bioscience Core Lab for their assistance with sequencing.Publisher
Springer NatureJournal
The ISME JournalPubMed ID
33649556Additional Links
http://www.nature.com/articles/s41396-021-00931-zRelations
Is Supplemented By:- [Bioproject]
Title: Suakin deep metagenomes. Publication Date: 2021-05-04. bioproject: PRJNA593704 Handle: 10754/669200
ae974a485f413a2113503eed53cd6c53
10.1038/s41396-021-00931-z
Scopus Count
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