Fine-scale metabolic discontinuity in a stratified prokaryote microbiome of a Red Sea deep halocline
Calleja Cortes, Maria de Lluch
Moran, Xose Anxelu G.
KAUST DepartmentBiological and Environmental Science and Engineering (BESE) Division
Extreme Systems Microbiology Lab
Marine Science Program
Microbial oceanography Research Group
Red Sea Research Center (RSRC)
Online Publication Date2021-03-01
Print Publication Date2021-08
Embargo End Date2021-09-01
Permanent link to this recordhttp://hdl.handle.net/10754/667877
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AbstractAbstractDeep-sea hypersaline anoxic basins are polyextreme environments in the ocean’s interior characterized by the high density of brines that prevents mixing with the overlaying seawater, generating sharp chemoclines and redoxclines up to tens of meters thick that host a high concentration of microbial communities. Yet, a fundamental understanding of how such pycnoclines shape microbial life and the associated biogeochemical processes at a fine scale, remains elusive. Here, we applied high-precision sampling of the brine–seawater transition interface in the Suakin Deep, located at 2770 m in the central Red Sea, to reveal previously undocumented fine-scale community structuring and succession of metabolic groups along a salinity gradient only 1 m thick. Metagenomic profiling at a 10-cm-scale resolution highlighted spatial organization of key metabolic pathways and corresponding microbial functional units, emphasizing the prominent role and significance of salinity and oxygen in shaping their ecology. Nitrogen cycling processes are especially affected by the redoxcline with ammonia oxidation processes being taxa and layers specific, highlighting also the presence of novel microorganisms, such as novel Thaumarchaeota and anammox, adapted to the changing conditions of the chemocline. The findings render the transition zone as a critical niche for nitrogen cycling, with complementary metabolic networks, in turn underscoring the biogeochemical complexity of deep-sea brines.
CitationMichoud, G., Ngugi, D. K., Barozzi, A., Merlino, G., Calleja, M. L., Delgado-Huertas, A., … Daffonchio, D. (2021). Fine-scale metabolic discontinuity in a stratified prokaryote microbiome of a Red Sea deep halocline. The ISME Journal. doi:10.1038/s41396-021-00931-z
SponsorsThis research was supported by King Abdullah University of Science and Technology through baseline funding and Red Sea Research Center competitive fund to DD. We thank the crew of R/V Thuwal and CMOR for their support in sampling the brines, especially Brian Hession and Thomas Hoover. We also thank personal of the KAUST Bioscience Core Lab for their assistance with sequencing.
JournalThe ISME Journal
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