Bioprospecting of Novel Extremozymes From Prokaryotes-The Advent of Culture-Independent Methods.
KAUST DepartmentKAUST Catalysis Center (KCC), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
Biological and Environmental Sciences and Engineering (BESE) Division
Office of the VP
Chemical Science Program
KAUST Catalysis Center (KCC)
Physical Science and Engineering (PSE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/667815
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AbstractExtremophiles are remarkable organisms that thrive in the harshest environments on Earth, such as hydrothermal vents, hypersaline lakes and pools, alkaline soda lakes, deserts, cold oceans, and volcanic areas. These organisms have developed several strategies to overcome environmental stress and nutrient limitations. Thus, they are among the best model organisms to study adaptive mechanisms that lead to stress tolerance. Genetic and structural information derived from extremophiles and extremozymes can be used for bioengineering other nontolerant enzymes. Furthermore, extremophiles can be a valuable resource for novel biotechnological and biomedical products due to their biosynthetic properties. However, understanding life under extreme conditions is challenging due to the difficulties of in vitro cultivation and observation since > 99% of organisms cannot be cultivated. Consequently, only a minor percentage of the potential extremophiles on Earth have been discovered and characterized. Herein, we present a review of culture-independent methods, sequence-based metagenomics (SBM), and single amplified genomes (SAGs) for studying enzymes from extremophiles, with a focus on prokaryotic (archaea and bacteria) microorganisms. Additionally, we provide a comprehensive list of extremozymes discovered via metagenomics and SAGs.
CitationSysoev, M., Grötzinger, S. W., Renn, D., Eppinger, J., Rueping, M., & Karan, R. (2021). Bioprospecting of Novel Extremozymes From Prokaryotes—The Advent of Culture-Independent Methods. Frontiers in Microbiology, 12. doi:10.3389/fmicb.2021.630013
SponsorsThe research reported in this publication was supported by funding from the King Abdullah University of Science and Technology (KAUST).
We are thankful to research interns Mayar A. Elbaz and Alvin Huang for their valuable inputs. Figure 1 was created using resources from svgrepo.com and Flaticon.com.
PublisherFrontiers Media SA
JournalFrontiers in microbiology
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