Gold nanostructured laser-scribed graphene: A new electrochemical biosensing platform for potential point-of-care testing of disease biomarkers
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2023-02-01
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ArticleAuthors
Rauf, Sakandar
Lahcen, Abdellatif Ait
Aljedaibi, Abdulrahman
Beduk, Tutku
Ilton de Oliveira Filho, José
Salama, Khaled N.

KAUST Department
Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) DivisionElectrical Engineering
Electrical Engineering Program
Material Science and Engineering Program
Physical Science and Engineering (PSE) Division
Sensors Lab
Sensors Lab, Advanced Membranes & Porous Materials Centre (AMPMC). Computer, Electrical and Mathematical Sciences and Engineering (CEMSE) Division, King Abdullah University of Science and Technology (KAUST), Thuwal, 23955-6900, Saudi Arabia.
Date
2021-02Embargo End Date
2023-02-01Submitted Date
2020-11-17Permanent link to this record
http://hdl.handle.net/10754/667684
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Show full item recordAbstract
Improvements in the Laser-scribed graphene (LSG)-based electrodes are critical to overcoming limitations of bare LSG electrodes in terms of sensitivity, direct immobilization of detection probes for biosensor fabrication, and ease of integration with point-of-care (POC) devices. Herein, we introduce a new class of nanostructured gold modified LSG (LSG-AuNS) electrochemical sensing system comprising LSG-AuNS working electrode, LSG reference, and LSG counter electrode. LSG-AuNS electrodes are realized by electrodeposition of gold chloride (HAuCl4) solution, which gave ∼2-fold enhancement in sensitivity and electrocatalytic activity compared to bare LSG electrode and commercially available screen-printed gold electrode (SPAuE). We demonstrate LSG-AuNS electrochemical aptasensor for detecting human epidermal growth factor receptor 2 (Her-2) with a limit of detection (LOD) of 0.008 ng/mL and a linear range of 0.1-200 ng/mL. LSG-AuNS-aptasensor can easily detect different concentrations of Her-2 spiked in undiluted human serum. Finally, to show the LSG-AuNS sensor system's potential to develop POC biosensor devices, we integrated LSG-AuNS electrodes with a handheld electrochemical system operated using a custom-developed mobile application.Citation
Rauf, S., Lahcen, A. A., Aljedaibi, A., Beduk, T., Ilton de Oliveira Filho, J., & Salama, K. N. (2021). Gold nanostructured laser-scribed graphene: A new electrochemical biosensing platform for potential point-of-care testing of disease biomarkers. Biosensors and Bioelectronics, 113116. doi:10.1016/j.bios.2021.113116Sponsors
The authors would like to express their acknowledgements to the financial support of funding from King Abdullah University of Science and Technology (KAUST), Saudi Arabia. Also, we thank the KAUST Sensor Initiative and KAUST visiting student program for supporting this work.Publisher
Elsevier BVJournal
Biosensors and BioelectronicsAdditional Links
https://linkinghub.elsevier.com/retrieve/pii/S0956566321001536ae974a485f413a2113503eed53cd6c53
10.1016/j.bios.2021.113116