Almulhim, Fatimah F.
Emwas, Abdul-Hamid M.
Atreya, Hanudatta S.
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/667673
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AbstractNMR-based metabolomics, which emerged along with mass spectrometry techniques, is the preferred method for studying metabolites in medical research and food industries. However, NMR techniques suffer from inherently low sensitivity, regardless of their superior reproducibility. To overcome this, we made two beneficial modifications: we detuned the probe to reach a position called “Spin Noise Tuning Optimum” (SNTO), and we replaced the conventional cylindrical 5 mm NMR tube with an electric field component-optimized shaped tube. We found that concerted use of both modifications can increase the sensitivity (signal to noise ratio per unit volume) and detection of metabolites and decrease the measurement time by order of magnitude. In this study, we demonstrate and discuss the achieved signal enhancement of metabolites on model non-human (bovine serum, amino acid standard mixture) and human urine samples.
CitationChandra, K., Al-Harthi, S., Sukumaran, S., Almulhim, F., Emwas, A.-H., Atreya, H. S., … Jaremko, M. (2021). NMR-based metabolomics with enhanced sensitivity. RSC Advances, 11(15), 8694–8700. doi:10.1039/d1ra01103k
SponsorsThe authors would like to thank the Imaging and Characterization Core Lab at the King Abdullah University of Science and Technology (KAUST) for access to the NMR facilities. KC acknowledges the NMR facilities of NMR Research Centre, Indian Institute of Science, and DST, India. This publication is based on work supported by KAUST Smart Health Initiative grants (SHI REI 4447) (LJ and MJ) and through baseline-funds (LJ and MJ).
PublisherRoyal Society of Chemistry (RSC)
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