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dc.contributor.authorKim, Daehyung
dc.contributor.authorbiasio, Alfredo D.
dc.contributor.authorGonzalez-Magaña, Amaia
dc.contributor.authorBu, Gayun
dc.contributor.authorRashid, Fahad
dc.contributor.authorHamdan, Samir
dc.contributor.authorBlanco, Francisco
dc.contributor.authorLee Lee, Jong-Bong
dc.date.accessioned2021-02-24T07:37:25Z
dc.date.available2021-02-24T07:37:25Z
dc.date.issued2020-02
dc.identifier.citationKim, D., biasio, A. D., Gonzalez-Magaña, A., Bu, G., Rashid, F., Hamdan, S., … Lee Lee, J.-B. (2020). Single-Molecule Imaging of PAF15-PCNA using DNA Skybridge. Biophysical Journal, 118(3), 71a. doi:10.1016/j.bpj.2019.11.563
dc.identifier.issn0006-3495
dc.identifier.doi10.1016/j.bpj.2019.11.563
dc.identifier.urihttp://hdl.handle.net/10754/667644
dc.description.abstractProliferating cell nuclear antigen (PCNA) behaves as a sliding clamp that increases the processivity of eukaryotic and archea DNA polymerases through a physical interaction. Upon DNA damage during DNA replication, PCNA-associated factor 15 (PAF15) in complex with PCNA plays an important role in switching from a replicative polymerase to a translesion synthesis polymerase that is capable of bypassing nucleotide-lesions. Using the DNA skybridge that is a surface-condition independent and high-throughput single-molecule optical imaging platform, we monitored the diffusive motion of the PCNA-PAF15 complex on DNA molecules. We found that only one PAF15 molecule is associated with PCNA at physiological salt conditions, which results in slowing the diffusion of PCNA by up to 20 times and significantly stabilizing PCNA on DNA. The PCNA drag is likely to facilitate the switching of DNA polymerases. Taken together with the previous co-crystal structure of PCNA with a PAF15 fragment, our results propose a PAF15-PCNA conformation where the central domain of PAF15 interacts with the front face of PCNA and its N-terminal domain passes inside the PCNA ring.
dc.publisherElsevier BV
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S0006349519314924
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Biophysical Journal. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biophysical Journal, [118, 3, (2020-02)] DOI: 10.1016/j.bpj.2019.11.563 . © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleSingle-Molecule Imaging of PAF15-PCNA using DNA Skybridge
dc.typePresentation
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentLaboratory of DNA Replication and Recombination
dc.identifier.journalBiophysical Journal
dc.rights.embargodate2021-02-01
dc.eprint.versionPost-print
dc.contributor.institutionPhysics, Pohang University of Science and Technology, Pohang, Republic of Korea.
dc.contributor.institutionUniversity of Leicester, Leicester, United Kingdom.
dc.contributor.institutionCentro de Inv Cooperativa en Biociencias, Derio, Spain.
dc.contributor.institutionIkerbasque (Basque Foundation for Science), Bilbo, Spain.
dc.contributor.institutionSchool of Interdisciplinary Bioscience & Bioengineering, Pohang University of Science and Technology, Pohang, Republic of Korea.
dc.identifier.volume118
dc.identifier.issue3
dc.identifier.pages71a
kaust.personRashid, Fahad
kaust.personHamdan, Samir


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