A Multi-OMICs Approach Sheds Light on the Higher Yield Phenotype and Enhanced Abiotic Stress Tolerance in Tobacco Lines Expressing the Carrot lycopene β-cyclase1 Gene
AuthorsMoreno, Juan C
Sokolowska, Ewelina M
KAUST DepartmentCenter for Desert Agriculture
Permanent link to this recordhttp://hdl.handle.net/10754/667621
MetadataShow full item record
AbstractRecently, we published a set of tobacco lines expressing the Daucus carota (carrot) DcLCYB1 gene with accelerated development, increased carotenoid content, photosynthetic efficiency, and yield. Because of this development, DcLCYB1 expression might be of general interest in crop species as a strategy to accelerate development and increase biomass production under field conditions. However, to follow this path, a better understanding of the molecular basis of this phenotype is essential. Here, we combine OMICs (RNAseq, proteomics, and metabolomics) approaches to advance our understanding of the broader effect of LCYB expression on the tobacco transcriptome and metabolism. Upon DcLCYB1 expression, the tobacco transcriptome (~2,000 genes), proteome (~700 proteins), and metabolome (26 metabolites) showed a high number of changes in the genes involved in metabolic processes related to cell wall, lipids, glycolysis, and secondary metabolism. Gene and protein networks revealed clusters of interacting genes and proteins mainly involved in ribosome and RNA metabolism and translation. In addition, abiotic stress-related genes and proteins were mainly upregulated in the transgenic lines. This was well in line with an enhanced stress (high light, salt, and H2O2) tolerance response in all the transgenic lines compared with the wild type. Altogether, our results show an extended and coordinated response beyond the chloroplast (nucleus and cytosol) at the transcriptome, proteome, and metabolome levels, supporting enhanced plant growth under normal and stress conditions. This final evidence completes the set of benefits conferred by the expression of the DcLCYB1 gene, making it a very promising bioengineering tool to generate super crops.
CitationMoreno, J. C., Martinez-Jaime, S., Kosmacz, M., Sokolowska, E. M., Schulz, P., Fischer, A., … Skirycz, A. (2021). A Multi-OMICs Approach Sheds Light on the Higher Yield Phenotype and Enhanced Abiotic Stress Tolerance in Tobacco Lines Expressing the Carrot lycopene β-cyclase1 Gene. Frontiers in Plant Science, 12. doi:10.3389/fpls.2021.624365
SponsorsWe want to thank Prof. Lothar Willmitzer for support and discussion, Änne Michaelis for technical assistance, Dr. Michael Tillich and Dr. Ralph Bock for providing access to the POTbaseMS for proteomics analysis, and the Max-Planck-Institut für Molekulare Pflanzenphysiologie Green Team for plant cultivation.
PublisherFrontiers Media SA
JournalFrontiers in plant science
Except where otherwise noted, this item's license is described as This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
- Lycopene β-cyclase expression influences plant physiology, development, and metabolism in tobacco plants.
- Authors: Kössler S, Armarego-Marriott T, Tarkowská D, Turečková V, Agrawal S, Mi J, de Souza LP, Schöttler MA, Schadach A, Fröhlich A, Bock R, Al-Babili S, Ruf S, Sampathkumar A, Moreno JC
- Issue date: 2021 Mar 29
- Increased Nicotiana tabacum fitness through positive regulation of carotenoid, gibberellin and chlorophyll pathways promoted by Daucus carota lycopene β-cyclase (Dclcyb1) expression.
- Authors: Moreno JC, Cerda A, Simpson K, Lopez-Diaz I, Carrera E, Handford M, Stange C
- Issue date: 2016 Apr
- Expression of a carotenogenic gene allows faster biomass production by redesigning plant architecture and improving photosynthetic efficiency in tobacco.
- Authors: Moreno JC, Mi J, Agrawal S, Kössler S, Turečková V, Tarkowská D, Thiele W, Al-Babili S, Bock R, Schöttler MA
- Issue date: 2020 Sep
- Levels of lycopene β-cyclase 1 modulate carotenoid gene expression and accumulation in Daucus carota.
- Authors: Moreno JC, Pizarro L, Fuentes P, Handford M, Cifuentes V, Stange C
- Issue date: 2013
- <i>Daucus carota DcPSY2</i> and <i>DcLCYB1</i> as Tools for Carotenoid Metabolic Engineering to Improve the Nutritional Value of Fruits.
- Authors: Arias D, Arenas-M A, Flores-Ortiz C, Peirano C, Handford M, Stange C
- Issue date: 2021