• Login
    View Item 
    •   Home
    • Research
    • Datasets
    • View Item
    •   Home
    • Research
    • Datasets
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguidePlumX LibguideSubmit an Item

    Statistics

    Display statistics

    A novel multidrug resistant Klebsiella pneumoniae strain harboring three co-occurring colistin resistance genes

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Type
    Bioproject
    Dataset
    Authors
    Hala, Sharif
    Antony, C P
    Momin, Afaque Ahmad Imtiyaz
    Alshehri, M
    Ben Rached, Fathia
    Al-Ahmadi, G
    Zakri, S
    Baadhaim, M
    Alsaedi, A
    Thaqafi, O A Al
    Arold, Stefan T.
    Al-Amri, A
    Pain, Arnab
    KAUST Department
    Pathogen Genomics Laboratory, Division of Biological and Environmental Sciences and Engineering (BESE), Thuwal, Makkah, Saudi Arabia.
    Red Sea Research Centre, Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Saudi Arabia.
    Date
    2020-01-10
    Permanent link to this record
    http://hdl.handle.net/10754/667569
    
    Metadata
    Show full item record
    Abstract
    Colistin represents the last-resort antibiotics used for treating carbapenem-resistant Enterobacteriaceae-related infections. Colistin resistance has been reported in Saudi Arabia in multidrug resistant (MDR) Gram-negativebacteria in up to 40% of the cases. Though, only a handful of colistin resistance cases were genetically investigated due to the lack of an approved clinical microbiology method for resistance detection. In this study, we aimed tocharacterize and survey the presence of mobilized colistin resistance (mcr) genes in MDR Klebsiella pneumoniae clinical isolates using whole-genome sequencing (WGS) as part of hospital-wide surveillance for antimicrobial resistance genes in Gram-negative MDR bacterial pathogen isolates (n=1,633) during 2014-2018 in Saudi Arabia. One of the isolates (NGKP-54) was cultured from the sputum of a female patient with pneumonia in her late-60s who was being treated for Non-Small-Cell Lung Carcinoma in 2015. We produced the complete genome of theNGKP-54 strain using a combination of short and long reads WGS and in silico multilocus sequence typing (MLST). The NGKP-54 isolate was assigned a novel sequence type (ST3513) that is a single-locus variant of ST1425. The ST1425 variant has been reported in the Middle East and known to be involved in the production of carbapenemases. The NGKP-54 isolate was resistant to aminoglycoside, cephalosporin, and fluoroquinolone,as determined by conventional microbiological protocols and colistin broth microdilution (BMD). The NGKP-54strain conferred resistance to colistin with MIC of 4 μg/ml. Analysis of the complete genome revealed the presence of three colistin-resistance determining genes including mcr-1, mcr-8, and a partial mcr-8 gene. We observed that allthree mcr-genes contributed to the colistin resistance of NGKP-54 in complementation experiments. Expression of mcr-1 and mcr-8 genes in E. coli DH5α independently conferred colistin resistance at higherconcentration than the original NGKP-54 isolate. Our phylogenetic analysis of all mcr variants revealed MCR-8 and MCR-5 are the closest orthologues to EptA. We have identified for the first time, the presence of mcr-8 in a colistinresistant K. pneumoniae strain in Saudi Arabia and this is the first global report of the co-occurrence of three mcr-encoding genes in the same strain. Saudi Arabia is a hub for annual mass-gathering events, thus increasing the chances that multi-resistant K. pneumoniae can potentially spread globally unless closely monitored for the presence of resistant-determinant loci using WGS-based protocols.
    Publisher
    NCBI
    Additional Links
    https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJEB36000
    Relations
    Is Supplement To:
    • [Article]
      Hala, S., Antony, C. P., Momin, A. A., Alshehri, M., Ben-Rached, F., Al-Ahmadi, G., … Pain, A. (2021). Co-occurrence of mcr-1 and mcr-8 genes in a multidrug-resistant Klebsiella pneumoniae from a 2015 clinical isolate. International Journal of Antimicrobial Agents, 106303. doi:10.1016/j.ijantimicag.2021.106303. DOI: 10.1016/j.ijantimicag.2021.106303 Handle: 10754/667533
    Collections
    Datasets

    entitlement

     
    DSpace software copyright © 2002-2021  DuraSpace
    Quick Guide | Contact Us | Send Feedback
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.