Show simple item record

dc.contributor.authorHala, Sharif
dc.contributor.authorAntony, C P
dc.contributor.authorMomin, Afaque Ahmad Imtiyaz
dc.contributor.authorAlshehri, M
dc.contributor.authorBen Rached, Fathia
dc.contributor.authorAl-Ahmadi, G
dc.contributor.authorZakri, S
dc.contributor.authorBaadhaim, M
dc.contributor.authorAlsaedi, A
dc.contributor.authorThaqafi, O A Al
dc.contributor.authorArold, Stefan T.
dc.contributor.authorAl-Amri, A
dc.contributor.authorPain, Arnab
dc.date.accessioned2021-02-21T13:45:23Z
dc.date.available2021-02-21T13:45:23Z
dc.date.issued2021-02-15
dc.date.submitted2020-06-23
dc.identifier.citationHala, S., Antony, C. P., Momin, A. A., Alshehri, M., Ben-Rached, F., Al-Ahmadi, G., … Pain, A. (2021). Co-occurrence of mcr-1 and mcr-8 genes in a multidrug-resistant Klebsiella pneumoniae from a 2015 clinical isolate. International Journal of Antimicrobial Agents, 106303. doi:10.1016/j.ijantimicag.2021.106303
dc.identifier.issn0924-8579
dc.identifier.pmid33592301
dc.identifier.doi10.1016/j.ijantimicag.2021.106303
dc.identifier.urihttp://hdl.handle.net/10754/667533
dc.description.abstractPolymyxin E (colistin) is among the last-resort antibiotics used to treat carbapenem-resistant Enterobacteriaceae-related infections [1]. Colistin had been discontinued for human use since it was found to be associated with nephrotoxicity and neurotoxicity, however, since the 1990s, clinicians were required to reconsider the clinical value of a modified version as a last resort antibiotic. Currently, single plasmid-mediated mobile colistin resistance (mcr) gene is recognized as a global threat, but multiple mcr gene combinations in the same pathogen are rarely identified [2]. Membrane binding domains in MCR proteins are necessary for membrane charge modifications involved in the colistin resistance mechanism [3]. Wang et al. discussed the first mcr-8 containing isolate of Klebsiella pneumoniae (KP) with a high minimum inhibitory concentration (MIC) to colistin in a Chinese hospital in 2016, which was prior to the discovery of the mcr-8 gene in 2017 from a pig farm in China related to colistin usage in animals [3]. Typically, mcr-variants are investigated through molecular identification tools or next-generation sequencing (NGS), which is not routinely implemented in clinical molecular biology laboratories. Such studies identified a close evolutionary relationship between mcr and phosphoethanolamine transferase (eptA) of Neisseria that is known to induce colistin resistance [3]. Multiple variants of mcr genes including co-occurrences in the same bacterial strain in the clinic has been identified through epidemiological surveillance of disease-causing pathogens. Continuous monitoring and identification of clinical cases that harbor unique mcr-variants is essential for understanding and monitoring colistin resistant bacteria. In this study, we identified a unique combination of colistin-resistance genes in a multidrug-resistant (MDR) KP clinical isolate and explored the role of the variant mcr genes in colistin resistance.
dc.description.sponsorshipWe wish to thank members of the Infectious Disease Department and the Pathology Laboratory at King Khaled hospital in the Ministry of the National Guards – Health Affairs Western region for their support of our research. We also wish to acknowledge the Bioscience core laboratory at KAUST for their help with the sequencing operations. We thank Malak Haidar from KAUST Pathogen Genomics Laboratory for the technical help. We also acknowledge help from the team of curators at the Institut Pasteur MLST system (Paris, France) for importing novel alleles, profiles, and/or isolates at http://bigsdb.pasteur.fr.
dc.description.sponsorshipThe research reported in this publication was supported by the King Abdullah University of Science and Technology (KAUST) through the baseline fund BAS/1/1020-01-01 to AP and BAS/1/1056-01-01 to STA, and the Award No. URF/1/1976-25 from the Office of Sponsored Research (OSR).
dc.publisherElsevier BV
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S0924857921000339
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in International journal of antimicrobial agents. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in International journal of antimicrobial agents, [, , (2021-02-16)] DOI: 10.1016/j.ijantimicag.2021.106303 . © 2021. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleCo-occurrence of mcr-1 and mcr-8 genes in a multidrug-resistant Klebsiella pneumoniae from a 2015 clinical isolate.
dc.typeArticle
dc.contributor.departmentBiological and Environmental Science and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.contributor.departmentComputational Bioscience Research Center (CBRC)
dc.contributor.departmentComputational Bioscience Research Centre (CBRC), Division of Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Makkah, Saudi Arabia.
dc.contributor.departmentPathogen Genomics Laboratory
dc.contributor.departmentStructural Biology and Engineering
dc.identifier.journalInternational journal of antimicrobial agents
dc.rights.embargodate2022-02-16
dc.eprint.versionPost-print
dc.contributor.institutionKing Saud bin Abdulaziz University for Health Sciences, Jeddah, Makkah, Saudi Arabia.
dc.contributor.institutionKing Abdullah International Medical Research Centre, Jeddah, Makkah, Saudi Arabia.
dc.contributor.institutionMinistry of National Guard Health Affairs, Jeddah Makkah, Saudi Arabia.
dc.contributor.institutionGlobal Institution for Collaborative Research and Education (GI-CoRE), Hokkaido University, N20 W10 Kita-ku, Sapporo, Japan.
dc.identifier.pages106303
kaust.personHala, Sharif
kaust.personAntony, C P
kaust.personAntony, C P
kaust.personMomin, A A
kaust.personBen Rached, Fathia
kaust.personArold, Stefan T.
kaust.personPain, Arnab
kaust.grant.numberBAS/1/1020-01-01
kaust.grant.numberBAS/1/1056-01-01
kaust.grant.numberURF/1/1976-25
dc.date.accepted2021-02-06
dc.relation.issupplementedbybioproject:PRJEB36000
dc.relation.issupplementedbyDOI:10.5281/zenodo.3752068
refterms.dateFOA2021-02-21T13:48:32Z
display.relations<b>Is Supplemented By:</b><br/> <ul><li><i>[Bioproject]</i> <br/> Title: A novel multidrug resistant Klebsiella pneumoniae strain harboring three co-occurring colistin resistance genes. Publication Date: 2020-01-10. bioproject: <a href="https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJEB36000" >PRJEB36000</a> Handle: <a href="http://hdl.handle.net/10754/667569" >10754/667569</a></a></li><li><i>[Dataset]</i> <br/> Hala, S., Antony, C. P., Momin, A. A., Alshehri, M., Ben-Rached, F., Al-Ahmadi, G., Zakri, S., Baadhaim, M., Alsaedi, A., Al Thaqafi, O. A., Arold, S. T., Al-Amri, A., &amp; Pain, A. (2020). A novel multidrug-resistant Klebsiella pneumoniae strain harboring three co-occurring mcr colistin resistance genes. <i>Zenodo</i>. https://doi.org/10.5281/ZENODO.3752068. DOI: <a href="https://doi.org/10.5281/zenodo.3752068" >10.5281/zenodo.3752068</a> Handle: <a href="http://hdl.handle.net/10754/667864" >10754/667864</a></a></li></ul>
kaust.acknowledged.supportUnitcore laboratory
kaust.acknowledged.supportUnitOSR
kaust.acknowledged.supportUnitBaseline funds
dc.date.published-online2021-02-15
dc.date.published-print2021-03


Files in this item

Thumbnail
Name:
E-SSM-Sinica.pdf
Size:
1.451Mb
Format:
PDF
Description:
Accepted manuscript
Embargo End Date:
2022-02-16

This item appears in the following Collection(s)

Show simple item record