Co-occurrence of mcr-1 and mcr-8 genes in a multidrug-resistant Klebsiella pneumoniae from a 2015 clinical isolate.
Antony, C P
Momin, Afaque Ahmad Imtiyaz
Ben Rached, Fathia
Thaqafi, O A Al
Arold, Stefan T.
KAUST DepartmentBioscience Program
Biological and Environmental Sciences and Engineering (BESE) Division
Pathogen Genomics Laboratory, Division of Biological and Environmental Sciences and Engineering (BESE), Thuwal, Makkah, Saudi Arabia.
Red Sea Research Centre, Biological and Environmental Sciences and Engineering, King Abdullah University of Science and Technology, Saudi Arabia.
Computational Bioscience Research Centre (CBRC), Division of Biological and Environmental Sciences and Engineering (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Makkah, Saudi Arabia.
Computational Bioscience Research Center (CBRC)
Embargo End Date2022-02-16
Permanent link to this recordhttp://hdl.handle.net/10754/667533
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AbstractPolymyxin E (colistin) is among the last-resort antibiotics used to treat carbapenem-resistant Enterobacteriaceae-related infections . Colistin had been discontinued for human use since it was found to be associated with nephrotoxicity and neurotoxicity, however, since the 1990s, clinicians were required to reconsider the clinical value of a modified version as a last resort antibiotic. Currently, single plasmid-mediated mobile colistin resistance (mcr) gene is recognized as a global threat, but multiple mcr gene combinations in the same pathogen are rarely identified . Membrane binding domains in MCR proteins are necessary for membrane charge modifications involved in the colistin resistance mechanism . Wang et al. discussed the first mcr-8 containing isolate of Klebsiella pneumoniae (KP) with a high minimum inhibitory concentration (MIC) to colistin in a Chinese hospital in 2016, which was prior to the discovery of the mcr-8 gene in 2017 from a pig farm in China related to colistin usage in animals . Typically, mcr-variants are investigated through molecular identification tools or next-generation sequencing (NGS), which is not routinely implemented in clinical molecular biology laboratories. Such studies identified a close evolutionary relationship between mcr and phosphoethanolamine transferase (eptA) of Neisseria that is known to induce colistin resistance . Multiple variants of mcr genes including co-occurrences in the same bacterial strain in the clinic has been identified through epidemiological surveillance of disease-causing pathogens. Continuous monitoring and identification of clinical cases that harbor unique mcr-variants is essential for understanding and monitoring colistin resistant bacteria. In this study, we identified a unique combination of colistin-resistance genes in a multidrug-resistant (MDR) KP clinical isolate and explored the role of the variant mcr genes in colistin resistance.
CitationHala, S., Antony, C. P., Momin, A. A., Alshehri, M., Ben-Rached, F., Al-Ahmadi, G., … Pain, A. (2021). Co-occurrence of mcr-1 and mcr-8 genes in a multidrug-resistant Klebsiella pneumoniae from a 2015 clinical isolate. International Journal of Antimicrobial Agents, 106303. doi:10.1016/j.ijantimicag.2021.106303
SponsorsWe wish to thank members of the Infectious Disease Department and the Pathology Laboratory at King Khaled hospital in the Ministry of the National Guards – Health Affairs Western region for their support of our research. We also wish to acknowledge the Bioscience core laboratory at KAUST for their help with the sequencing operations. We thank Malak Haidar from KAUST Pathogen Genomics Laboratory for the technical help. We also acknowledge help from the team of curators at the Institut Pasteur MLST system (Paris, France) for importing novel alleles, profiles, and/or isolates at http://bigsdb.pasteur.fr.
The research reported in this publication was supported by the King Abdullah University of Science and Technology (KAUST) through the baseline fund BAS/1/1020-01-01 to AP and BAS/1/1056-01-01 to STA, and the Award No. URF/1/1976-25 from the Office of Sponsored Research (OSR).
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