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dc.contributor.authorGhezzi, D.
dc.contributor.authorSauro, F.
dc.contributor.authorColumbu, A.
dc.contributor.authorCarbone, C.
dc.contributor.authorHong, Pei-Ying
dc.contributor.authorVergara, F.
dc.contributor.authorDe Waele, J.
dc.contributor.authorCappelletti, M.
dc.date.accessioned2021-02-21T06:55:16Z
dc.date.available2021-02-21T06:55:16Z
dc.date.issued2021-02-16
dc.date.submitted2020-04-20
dc.identifier.citationGhezzi, D., Sauro, F., Columbu, A., Carbone, C., Hong, P.-Y., Vergara, F., … Cappelletti, M. (2021). Transition from unclassified Ktedonobacterales to Actinobacteria during amorphous silica precipitation in a quartzite cave environment. Scientific Reports, 11(1). doi:10.1038/s41598-021-83416-5
dc.identifier.issn2045-2322
dc.identifier.pmid33594175
dc.identifier.doi10.1038/s41598-021-83416-5
dc.identifier.urihttp://hdl.handle.net/10754/667514
dc.description.abstractAbstractThe orthoquartzite Imawarì Yeuta cave hosts exceptional silica speleothems and represents a unique model system to study the geomicrobiology associated to silica amorphization processes under aphotic and stable physical–chemical conditions. In this study, three consecutive evolution steps in the formation of a peculiar blackish coralloid silica speleothem were studied using a combination of morphological, mineralogical/elemental and microbiological analyses. Microbial communities were characterized using Illumina sequencing of 16S rRNA gene and clone library analysis of carbon monoxide dehydrogenase (coxL) and hydrogenase (hypD) genes involved in atmospheric trace gases utilization. The first stage of the silica amorphization process was dominated by members of a still undescribed microbial lineage belonging to the Ktedonobacterales order, probably involved in the pioneering colonization of quartzitic environments. Actinobacteria of the Pseudonocardiaceae and Acidothermaceae families dominated the intermediate amorphous silica speleothem and the final coralloid silica speleothem, respectively. The atmospheric trace gases oxidizers mostly corresponded to the main bacterial taxa present in each speleothem stage. These results provide novel understanding of the microbial community structure accompanying amorphization processes and of coxL and hypD gene expression possibly driving atmospheric trace gases metabolism in dark oligotrophic caves.
dc.description.sponsorshipWe acknowledge the Rector Prof. Francesco Ubertini, the Vice-Rector for Research Prof. A. Rotolo and the Governing Academic Bodies of the University of Bologna (UNIBO) for the financial support of the research project and the PhD scholarship for Daniele Ghezzi. Funding for part of the microbial analyses was provided by Europlanet 2020 17-EPN3-026 grant. Europlanet 2020 RI has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 654208. Our gratitude also goes to Prof. Christine Moissl-Eichinger, Dr. Kaisa Koskinen, Dr. Alexander Mahnert, Dr. Slave Trajanoski and Dr. Manuela Pausan at the Medical University of Graz (Austria) for their help with sample preparation for Illumina sequencing, qPCR and data analysis. We thank Prof. Leonardo Piccini for his contribution in the sample collection. Many thanks to Dr. Hosam Zowawi for supporting contacts with KAUST and for further advancement of the research project in the last years, and Dr. Laura Negretti for technical assistance in SEM and FESEM analyses. We acknowledge the agencies and associations involved in granting the permit for the speleological expeditions and samples collection: Instituto National de Parques and the patronage of the Government of Bolivar State from Venezuela, the Embassy of the Bolivarian Republic of Venezuela in Italy. Finally, we are grateful to Dr. Andrea Firrincieli for his support about theoretical aspects of EMIRGE algorithm and the UNIBO students Lisa Foschi, Andrea Gozzi and Alessandro Caprini for their precious contribution in the experimental work. This article has been developed in the framework of the Rolex Award of Enterprise supporting Francesco Sauro in research and exploration on the tepui highlands of South America.
dc.publisherSpringer Nature
dc.relation.urlhttp://www.nature.com/articles/s41598-021-83416-5
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.titleTransition from unclassified Ktedonobacterales to Actinobacteria during amorphous silica precipitation in a quartzite cave environment
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentEnvironmental Microbial Safety and Biotechnology Lab
dc.contributor.departmentEnvironmental Science and Engineering Program
dc.contributor.departmentWater Desalination and Reuse Research Center (WDRC)
dc.identifier.journalScientific Reports
dc.identifier.pmcidPMC7887251
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionDepartment of Pharmacy and Biotechnology, University of Bologna, 40126 Bologna, Italy.
dc.contributor.institutionLaboratory of NanoBiotechnology, IRCCS Istituto Ortopedico Rizzoli, 40136 Bologna, Italy.
dc.contributor.institutionDepartment of Biological Geological and Environmental Sciences, University of Bologna, 40126 Bologna, Italy.
dc.contributor.institutionLa Venta Geographic Explorations Association, 31100 Treviso, Italy.
dc.contributor.institutionTeraphosa Exploring Team, Puerto Ordaz, Venezuela.
dc.contributor.institutionDepartment of Earth, Environment and Life, University of Genoa, 16132 Genoa, Italy.
dc.identifier.volume11
dc.identifier.issue1
kaust.personHong, Pei-Ying
dc.date.accepted2021-01-27
refterms.dateFOA2021-02-21T06:56:04Z


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This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
Except where otherwise noted, this item's license is described as This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.