Insertion of PATC-rich C. elegans introns into synthetic transgenes by golden-gate-based cloning
Type
PreprintAuthors
Frøkjær-Jensen, Christian
KAUST Department
Biological and Environmental Science and Engineering (BESE) DivisionBioscience Program
Date
2021-02-15Permanent link to this record
http://hdl.handle.net/10754/667480
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Transgenes are particularly prone to epigenetic silencing in the C. elegans germline. Here, we describe a protocol to insert introns containing a class of non-coding DNA named Periodic An/Tn Clusters (PATCs) into synthetic transgenes. PATCs can protect transgenes from position-dependent silencing (Position Effect Variegation, PEV) and from silencing in simple extra-chromosomal arrays. Using a set of simple design rules, it is possible to routinely insert up to three PATC-rich introns into a synthetic transgene in a single reaction.Citation
Frøkjær-Jensen, C. (2021). Insertion of PATC-rich C. elegans introns into synthetic transgenes by golden-gate-based cloning. doi:10.21203/rs.3.pex-1253/v1Publisher
Research Square Platform LLCAdditional Links
https://protocolexchange.researchsquare.com/article/pex-1253/v1https://assets.researchsquare.com/files/pex-1253/v1/1e880176-df21-44f0-af57-2bc0a944835a.pdf
ae974a485f413a2113503eed53cd6c53
10.21203/rs.3.pex-1253/v1
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