Insertion of PATC-rich C. elegans introns into synthetic transgenes by golden-gate-based cloning
KAUST DepartmentBiological and Environmental Sciences and Engineering (BESE) Division
Permanent link to this recordhttp://hdl.handle.net/10754/667480
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AbstractTransgenes are particularly prone to epigenetic silencing in the C. elegans germline. Here, we describe a protocol to insert introns containing a class of non-coding DNA named Periodic An/Tn Clusters (PATCs) into synthetic transgenes. PATCs can protect transgenes from position-dependent silencing (Position Effect Variegation, PEV) and from silencing in simple extra-chromosomal arrays. Using a set of simple design rules, it is possible to routinely insert up to three PATC-rich introns into a synthetic transgene in a single reaction.
CitationFrøkjær-Jensen, C. (2021). Insertion of PATC-rich C. elegans introns into synthetic transgenes by golden-gate-based cloning. doi:10.21203/rs.3.pex-1253/v1
PublisherResearch Square Platform LLC
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