• Login
    View Item 
    •   Home
    • Office of Sponsored Research (OSR)
    • KAUST Funded Research
    • Publications Acknowledging KAUST Support
    • View Item
    •   Home
    • Office of Sponsored Research (OSR)
    • KAUST Funded Research
    • Publications Acknowledging KAUST Support
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Browse

    All of KAUSTCommunitiesIssue DateSubmit DateThis CollectionIssue DateSubmit Date

    My Account

    Login

    Quick Links

    Open Access PolicyORCID LibguideTheses and Dissertations LibguideSubmit an Item

    Statistics

    Display statistics

    Laser-induced graphene interdigitated electrodes for label-free or nanolabel-enhanced highly sensitive capacitive aptamer-based biosensors

    • CSV
    • RefMan
    • EndNote
    • BibTex
    • RefWorks
    Type
    Article
    Authors
    Yagati, Ajay Kumar cc
    Behrent, Arne
    Beck, Sebastian
    Rink, Simone
    Goepferich, Achim M. cc
    Min, Junhong cc
    Lee, Min-Ho cc
    Baeumner, Antje J. cc
    Date
    2020-09
    Embargo End Date
    2022-06-01
    Permanent link to this record
    http://hdl.handle.net/10754/667360
    
    Metadata
    Show full item record
    Abstract
    Highly porous laser-induced graphene (LIG) is easily generated in complex electrode configurations such as interdigitated electrodes (IDEs). Here, we demonstrate that their superior capacitive response at low frequencies can be exploited in affinity biosensors using thrombin aptamers as model biorecognition elements. Of specific interest was the effect of electrode surface area on capacitance detection, and the comparison between a label-free format and enhancement strategies afforded by carboxy group bearing polymeric nanoparticles or liposomes. Electrochemical impedance spectroscopy (EIS) was used to investigate the LIG performance and optimize the biosensor design. Interestingly, the label-free strategy performed extremely well and additional labels decreased the limit of detection or increased the sensitivity only minimally. It is assumed that the highly porous nature of the LIG structures dominates the capacitive response so that labels removed from the surface have only limited influence Also, while slight performance changes can be observed for smaller vs. larger electrode structures, the performance of a LIG IDE is reasonably independent of its size. In the end, a dynamic range of 5 orders of magnitude was obtained (0.01 nM–1000 nM) with a limit of detection as low as 0.12 pM. When measured in serum, this increased to 1.3 pM. The good reproducibility (relative standard deviation (RSD), 4.90%) and repeatability (RSD, 2.59%) and good long-term stability (>7 weeks at 4 °C) prove that a LIG-based capacitance sensor is an excellent choice for affinity-based biosensor. The ease-of-production, the simplicity of modification and the superior performance even in a label-free format indicate that LIG-based biosensors should be considered in point-of-care diagnostics in the future.
    Citation
    Yagati, A. K., Behrent, A., Beck, S., Rink, S., Goepferich, A. M., Min, J., … Baeumner, A. J. (2020). Laser-induced graphene interdigitated electrodes for label-free or nanolabel-enhanced highly sensitive capacitive aptamer-based biosensors. Biosensors and Bioelectronics, 164, 112272. doi:10.1016/j.bios.2020.112272
    Sponsors
    Authors sincerely thank Professor Reinhard Rachel and Mr. Marcel Simsek for providing technical help and assistance in SEM measurements and Ms.Vanessa Tomanek for her contributions to developing the CAD liposome model. This work was supported in part by the South Korean Ministry of Trade, Industry, and Energy (Grant no. P0005440), the South Korean Ministry of Environment (Grant no. 201901136), and the German Federal Ministry of Economic Affairs and Energy (Grant no. ZF4104802AW8). The laser-scriber device was made available through a grant by the King Abdullah University of Science and Technology (KAUST).
    Publisher
    Elsevier BV
    Journal
    Biosensors and Bioelectronics
    DOI
    10.1016/j.bios.2020.112272
    Additional Links
    https://linkinghub.elsevier.com/retrieve/pii/S0956566320302670
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.bios.2020.112272
    Scopus Count
    Collections
    Publications Acknowledging KAUST Support

    entitlement

     
    DSpace software copyright © 2002-2022  DuraSpace
    Quick Guide | Contact Us | KAUST University Library
    Open Repository is a service hosted by 
    Atmire NV
     

    Export search results

    The export option will allow you to export the current search results of the entered query to a file. Different formats are available for download. To export the items, click on the button corresponding with the preferred download format.

    By default, clicking on the export buttons will result in a download of the allowed maximum amount of items. For anonymous users the allowed maximum amount is 50 search results.

    To select a subset of the search results, click "Selective Export" button and make a selection of the items you want to export. The amount of items that can be exported at once is similarly restricted as the full export.

    After making a selection, click one of the export format buttons. The amount of items that will be exported is indicated in the bubble next to export format.