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dc.contributor.authorMonachino, Enrico
dc.contributor.authorJergic, Slobodan
dc.contributor.authorLewis, Jacob S.
dc.contributor.authorXu, Zhi-Qiang
dc.contributor.authorLo, Allen T.Y.
dc.contributor.authorO’Shea, Valerie L.
dc.contributor.authorBerger, James M.
dc.contributor.authorDixon, Nicholas E.
dc.contributor.authorvan Oijen, Antoine M.
dc.date.accessioned2021-02-11T10:51:23Z
dc.date.available2021-02-11T10:51:23Z
dc.date.issued2020-07
dc.identifier.citationMonachino, E., Jergic, S., Lewis, J. S., Xu, Z.-Q., Lo, A. T. Y., O’Shea, V. L., … van Oijen, A. M. (2020). A Primase-Induced Conformational Switch Controls the Stability of the Bacterial Replisome. Molecular Cell, 79(1), 140–154.e7. doi:10.1016/j.molcel.2020.04.037
dc.identifier.issn1097-2765
dc.identifier.doi10.1016/j.molcel.2020.04.037
dc.identifier.urihttp://hdl.handle.net/10754/667353
dc.description.abstractRecent studies of bacterial DNA replication have led to a picture of the replisome as an entity that freely exchanges DNA polymerases and displays intermittent coupling between the helicase and polymerase(s). Challenging the textbook model of the polymerase holoenzyme acting as a stable complex coordinating the replisome, these observations suggest a role of the helicase as the central organizing hub. We show here that the molecular origin of this newly found plasticity lies in the 500-fold increase in strength of the interaction between the polymerase holoenzyme and the replicative helicase upon association of the primase with the replisome. By combining in vitro ensemble-averaged and single-molecule assays, we demonstrate that this conformational switch operates during replication and promotes recruitment of multiple holoenzymes at the fork. Our observations provide a molecular mechanism for polymerase exchange and offer a revised model for the replication reaction that emphasizes its stochasticity.
dc.description.sponsorshipWe are indebted to Karl Duderstadt and Christiaan Punter for ImageJ plugins, Yao Wang for providing protein reagents, Lisanne Spenkelink for development of and assistance with the sm-FRAP assays, and Harshad Ghodke for fruitful discussions. This work was supported by the Australian Research Council (grants DP150100956 and DP180100858 to A.M.v.O. and N.E.D. and Australian Laureate Fellowship FL140100027 to A.M.v.O.), King Abdullah University of Science and Technology, Saudi Arabia (grant OSR-2015-CRG4-2644 to N.E.D. and A.M.v.O), Nederlandse Organisatie voor Wetenschappelijk Onderzoek (grant 12CMCE03 to E.M.), and the National Institutes of Health (NIGMS grant R37-071747 to J.M.B.). Conceptualization, E.M. S.J. N.E.D. and A.M.v.O.; Methodology, E.M. S.J. J.S.L. N.E.D. and A.M.v.O.; Resources, E.M. S.J. J.S.L. Z.-Q.X. A.T.Y.L. and V.L.O.; Software, E.M.; Validation, Formal Analysis, & Writing – Original Draft, E.M. and S.J.; Investigation, E.M. S.J. and J.S.L.; Supervision, S.J. N.E.D. and A.M.v.O.; Writing – Review & Editing, E.M. S.J. J.M.B. N.E.D. and A.M.v.O.; Funding Acquisition, J.M.B. N.E.D. and A.M.v.O. The authors declare no competing interests.
dc.publisherElsevier BV
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S1097276520302793
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Molecular Cell. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Molecular Cell, [79, 1, (2020-07)] DOI: 10.1016/j.molcel.2020.04.037 . © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleA Primase-Induced Conformational Switch Controls the Stability of the Bacterial Replisome
dc.typeArticle
dc.identifier.journalMolecular Cell
dc.rights.embargodate2021-07-02
dc.eprint.versionPost-print
dc.contributor.institutionMolecular Horizons and School of Chemistry and Molecular Bioscience, University of Wollongong, and Illawarra Health and Medical Research Institute, Wollongong, NSW 2522, Australia
dc.contributor.institutionZernike Institute for Advanced Materials, University of Groningen, Groningen 9747, the Netherlands
dc.contributor.institutionDepartment of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA
dc.identifier.volume79
dc.identifier.issue1
dc.identifier.pages140-154.e7
kaust.grant.numberOSR-2015-CRG4-2644
dc.identifier.eid2-s2.0-85086359277
kaust.acknowledged.supportUnitOSR


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