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dc.contributor.authorTaylor, Raegyn B.
dc.contributor.authorHill, Bridgett N.
dc.contributor.authorBobbitt, Jonathan M.
dc.contributor.authorHering, Amanda S.
dc.contributor.authorBrooks, Bryan W.
dc.contributor.authorChambliss, C. Kevin
dc.date.accessioned2021-02-11T08:43:48Z
dc.date.available2021-02-11T08:43:48Z
dc.date.issued2020-05
dc.identifier.citationTaylor, R. B., Hill, B. N., Bobbitt, J. M., Hering, A. S., Brooks, B. W., & Chambliss, C. K. (2020). Suspect and non-target screening of acutely toxic Prymnesium parvum. Science of The Total Environment, 715, 136835. doi:10.1016/j.scitotenv.2020.136835
dc.identifier.issn0048-9697
dc.identifier.doi10.1016/j.scitotenv.2020.136835
dc.identifier.urihttp://hdl.handle.net/10754/667347
dc.description.abstractHarmful algal blooms (HABs) are increasing in frequency, magnitude, and duration around the world. Prymnesium parvum is a HAB species known to cause massive fish kills, but the toxin(s) it produces contributing to this acute toxicity to fish have not been confirmed. In the present study, a 2 × 2 factorial design was employed to examine influences of salinity (2.4 or 5 ppt) and nutrient limitation (f/2 or f/8) on P. parvum acute toxicity to fish and produced molecules. Acute toxicity (LC50) of these cultures, following a 48-h mortality assay, ranged from 10,213 to 96,816 cells mL−1. Non-targeted analysis was performed using liquid chromatography high-resolution mass spectrometry (LC-HRMS) to investigate compounds contributing to the differential toxicological responses. When P. parvum elicited toxicity to fish, suspect screening confirmed the presence of several prymnesins, and the peak area of PRM-A (3 Cl; prymnesin2aglycone) was significantly (p < 0.05) and positively related to acute toxicity. In addition, a non-targeted approach to highlighting peaks that differ between two chemical fingerprints was developed, termed a relative difference plot, and used to search for peaks co-varying with P. parvum induced acute toxicity to fish. Several peaks were highlighted along with the prymnesins identified through suspect screening when acute toxicity to fish was observed.
dc.description.sponsorshipThe authors acknowledge the Baylor University Mass Spectrometry Center (BU-MSC) and the Center for Reservoir and Aquatic Systems Research for support during this work. This research was partially supported by the National Institute of Environmental Health Sciences of the National Institutes of Health under award number 1P01ES028942 to BWB. Dr. Hering's work was supported in part by the King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) under award no: OSR-2015-CRG4-2582.
dc.publisherElsevier BV
dc.relation.urlhttps://linkinghub.elsevier.com/retrieve/pii/S0048969720303454
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Science of The Total Environment. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Science of The Total Environment, [715, , (2020-05)] DOI: 10.1016/j.scitotenv.2020.136835 . © 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleSuspect and non-target screening of acutely toxic Prymnesium parvum
dc.typeArticle
dc.identifier.journalScience of The Total Environment
dc.rights.embargodate2021-01-30
dc.eprint.versionPost-print
dc.contributor.institutionDepartment of Chemistry and Biochemistry, Baylor University, One Bear Place #97348, Waco, TX 76798, USA
dc.contributor.institutionDepartment of Environmental Science, Baylor University, One Bear Place #97266, Waco, TX 76798, USA
dc.contributor.institutionDepartment of Statistical Science, Baylor University, One Bear Place #97140, Waco, TX 76798, USA
dc.identifier.volume715
dc.identifier.pages136835
kaust.grant.numberOSR-2015-CRG4-2582.
dc.identifier.eid2-s2.0-85078660778
kaust.acknowledged.supportUnitOffice of Sponsored Research (OSR)


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