Suspect and non-target screening of acutely toxic Prymnesium parvum
AuthorsTaylor, Raegyn B.
Hill, Bridgett N.
Bobbitt, Jonathan M.
Hering, Amanda S.
Brooks, Bryan W.
Chambliss, C. Kevin
KAUST Grant NumberOSR-2015-CRG4-2582.
Embargo End Date2021-01-30
Permanent link to this recordhttp://hdl.handle.net/10754/667347
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AbstractHarmful algal blooms (HABs) are increasing in frequency, magnitude, and duration around the world. Prymnesium parvum is a HAB species known to cause massive fish kills, but the toxin(s) it produces contributing to this acute toxicity to fish have not been confirmed. In the present study, a 2 × 2 factorial design was employed to examine influences of salinity (2.4 or 5 ppt) and nutrient limitation (f/2 or f/8) on P. parvum acute toxicity to fish and produced molecules. Acute toxicity (LC50) of these cultures, following a 48-h mortality assay, ranged from 10,213 to 96,816 cells mL−1. Non-targeted analysis was performed using liquid chromatography high-resolution mass spectrometry (LC-HRMS) to investigate compounds contributing to the differential toxicological responses. When P. parvum elicited toxicity to fish, suspect screening confirmed the presence of several prymnesins, and the peak area of PRM-A (3 Cl; prymnesin2aglycone) was significantly (p < 0.05) and positively related to acute toxicity. In addition, a non-targeted approach to highlighting peaks that differ between two chemical fingerprints was developed, termed a relative difference plot, and used to search for peaks co-varying with P. parvum induced acute toxicity to fish. Several peaks were highlighted along with the prymnesins identified through suspect screening when acute toxicity to fish was observed.
CitationTaylor, R. B., Hill, B. N., Bobbitt, J. M., Hering, A. S., Brooks, B. W., & Chambliss, C. K. (2020). Suspect and non-target screening of acutely toxic Prymnesium parvum. Science of The Total Environment, 715, 136835. doi:10.1016/j.scitotenv.2020.136835
SponsorsThe authors acknowledge the Baylor University Mass Spectrometry Center (BU-MSC) and the Center for Reservoir and Aquatic Systems Research for support during this work. This research was partially supported by the National Institute of Environmental Health Sciences of the National Institutes of Health under award number 1P01ES028942 to BWB. Dr. Hering's work was supported in part by the King Abdullah University of Science and Technology (KAUST) Office of Sponsored Research (OSR) under award no: OSR-2015-CRG4-2582.
JournalScience of The Total Environment