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dc.contributor.authorDal Magro, Roberta
dc.contributor.authorVitali, Agostina
dc.contributor.authorFagioli, Stefano
dc.contributor.authorCasu, Alberto
dc.contributor.authorFalqui, Andrea
dc.contributor.authorFormicola, Beatrice
dc.contributor.authorTaiarol, Lorenzo
dc.contributor.authorCassina, Valeria
dc.contributor.authorMarrano, Claudia Adriana
dc.contributor.authorMantegazza, Francesco
dc.contributor.authorAnselmi-Tamburini, Umberto
dc.contributor.authorSommi, Patrizia
dc.contributor.authorRe, Francesca
dc.date.accessioned2021-02-11T06:18:52Z
dc.date.available2021-02-11T06:18:52Z
dc.date.issued2021-02-09
dc.date.submitted2021-01-11
dc.identifier.citationDal Magro, R., Vitali, A., Fagioli, S., Casu, A., Falqui, A., Formicola, B., … Re, F. (2021). Oxidative Stress Boosts the Uptake of Cerium Oxide Nanoparticles by Changing Brain Endothelium Microvilli Pattern. Antioxidants, 10(2), 266. doi:10.3390/antiox10020266
dc.identifier.issn2076-3921
dc.identifier.doi10.3390/antiox10020266
dc.identifier.urihttp://hdl.handle.net/10754/667335
dc.description.abstractVascular oxidative stress is considered a worsening factor in the progression of Alzheimer’s disease (AD). Increased reactive oxygen species (ROS) levels promote the accumulation of amyloid-β peptide (Aβ), one of the main hallmarks of AD. In turn, Aβ is a potent inducer of oxidative stress. In early stages of AD, the concomitant action of oxidative stress and Aβ on brain capillary endothelial cells was observed to compromise the blood–brain barrier functionality. In this context, antioxidant compounds might provide therapeutic benefits. To this aim, we investigated the antioxidant activity of cerium oxide nanoparticles (CNP) in human cerebral microvascular endothelial cells (hCMEC/D3) exposed to Aβ oligomers. Treatment with CNP (13.9 ± 0.7 nm in diameter) restored basal ROS levels in hCMEC/D3 cells, both after acute or prolonged exposure to Aβ. Moreover, we found that the extent of CNP uptake by hCMEC/D3 was +43% higher in the presence of Aβ. Scanning electron microscopy and western blot analysis suggested that changes in microvilli structures on the cell surface, under pro-oxidant stimuli (Aβ or H2O2), might be involved in the enhancement of CNP uptake. This finding opens the possibility to exploit the modulation of endothelial microvilli pattern to improve the uptake of anti-oxidant particles designed to counteract ROS-mediated cerebrovascular dysfunctions.
dc.description.sponsorshipPart of this work was funded by Fondazione Banca del Monte di Lombardia to Umberto Anselmi-Tamburini, by KAUST Baseline funding to Andrea Falqui, by the Italian Ministry of University and Research (MIUR)—Department of Excellence project PREMIA (PREcision MedIcine Approach: bringing biomarker research to clinic) to Claudia A. Marrano and by ATE-Fondo di Ateneo Università Milano-Bicocca (2018-ATE-0528) to Francesca Re.
dc.publisherMDPI AG
dc.relation.urlhttps://www.mdpi.com/2076-3921/10/2/266
dc.rightsThis article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license.
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleOxidative Stress Boosts the Uptake of Cerium Oxide Nanoparticles by Changing Brain Endothelium Microvilli Pattern
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentBioscience Program
dc.identifier.journalAntioxidants
dc.eprint.versionPublisher's Version/PDF
dc.contributor.institutionBioNanoMedicine Center NANOMIB, School of Medicine and Surgery, University of Milano-Bicocca, 20900 Monza, Italy.
dc.contributor.institutionDepartment of Chemistry, University of Pavia, 27100 Pavia, Italy.
dc.contributor.institutionHuman Physiology Unit, Department of Molecular Medicine, University of Pavia, 27100 Pavia, Italy.
dc.identifier.volume10
dc.identifier.issue2
dc.identifier.pages266
kaust.personCasu, Alberto
kaust.personFalqui, Andrea
dc.date.accepted2021-02-05
refterms.dateFOA2021-02-11T06:19:48Z
kaust.acknowledged.supportUnitBaseline funding


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