Show simple item record

dc.contributor.authorZheng, Xiongjie
dc.contributor.authorMi, Jianing
dc.contributor.authorDeng, Xiuxin
dc.contributor.authorAl-Babili, Salim
dc.date.accessioned2021-02-10T06:31:40Z
dc.date.available2021-02-10T06:31:40Z
dc.date.issued2021-02-05
dc.identifier.citationZheng, X., Mi, J., Deng, X., & Al-Babili, S. (2021). LC–MS-Based Profiling Provides New Insights into Apocarotenoid Biosynthesis and Modifications in Citrus Fruits. Journal of Agricultural and Food Chemistry. doi:10.1021/acs.jafc.0c06893
dc.identifier.issn0021-8561
dc.identifier.issn1520-5118
dc.identifier.pmid33543938
dc.identifier.doi10.1021/acs.jafc.0c06893
dc.identifier.urihttp://hdl.handle.net/10754/667307
dc.description.abstractApocarotenoids contribute to fruit color and aroma, which are critical quality and marketability attributes. Previously, we reported that the red peels of citrus fruits, which are characterized by higher expression levels of a <i>carotenoid cleavage dioxygenase</i> 4<i>b</i> (<i>CitCCD</i>4<i>b</i>) gene, accumulate higher levels of β-citraurin and β-citraurinene than yellow peels. Here, we identified and quantified 12 apocarotenoids, either volatile or nonvolatile, in citrus peel using liquid chromatography-mass spectrometry (LC-MS). Our results show that red peels contain also dramatically higher amounts of β-apo-8'-carotenal, crocetin dialdehyde known from saffron, β-citraurol, β-cyclocitral, and 3-OH-β-cyclocitral and up to about 17-fold higher levels of 3-OH-β-cyclocitral glucoside (picrocrocin isomer). The content of these apocarotenoids was also significantly increased in different <i>CitCCD</i>4<i>b</i>-overexpressing transgenic callus lines, compared with corresponding controls. Transient expression of <i>CitCCD</i>4<i>b</i> in <i>Nicotiana benthamiana</i> leaves resulted in a striking increase in the 3-OH-β-cyclocitral level and the accumulation of picrocrocin. Thus, our work reinforces the specific function of CitCCD4b in producing C<sub>10</sub> apocarotenoid volatiles and C<sub>30</sub> pigments in citrus peel and uncovers its involvement in the biosynthesis of picrocrocin, C<sub>20</sub> dialdehyde, and C<sub>30</sub> alcohol apocarotenoids, suggesting the potential of this enzyme in metabolic engineering of apocarotenoids and their derivatives.
dc.description.sponsorshipThis work was supported by baseline funding to S.A.-B. from King Abdullah University for Science and Technology (KAUST). The authors acknowledge the financial funding from the National Key Research and Development Program of China (2018YFD1000200) and the National Natural Science Foundation of China (Nos. 31930095, 31630065) to support X.D. of Huazhong Agricultural University (HZAU). The authors greatly appreciate Qitong Feng for his excellent technical assistance and Dr. Hendrik N.J. Kuijer, and Dr. Yagiz Alagoz for valuable comments
dc.publisherAmerican Chemical Society (ACS)
dc.relation.urlhttps://pubs.acs.org/doi/10.1021/acs.jafc.0c06893
dc.rightsThis document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/10.1021/acs.jafc.0c06893.
dc.subjectcitrus
dc.subjectLC−MS, CCD4
dc.subjectapocarotenoids
dc.subjectpicrocrocin
dc.subjectcrocetin dialdehyde
dc.subjectsaffron
dc.subjectβ-citraurol
dc.titleLC–MS-Based Profiling Provides New Insights into Apocarotenoid Biosynthesis and Modifications in Citrus Fruits
dc.typeArticle
dc.contributor.departmentBiological and Environmental Sciences and Engineering (BESE) Division
dc.contributor.departmentPlant Science
dc.contributor.departmentDesert Agriculture Initiative
dc.identifier.journalJournal of Agricultural and Food Chemistry
dc.rights.embargodate2022-02-05
dc.eprint.versionPost-print
dc.contributor.institutionKey Laboratory of Horticultural Plant Biology (Ministry of Education), Huazhong Agricultural University, Wuhan 430070, China
kaust.personZheng, Xiongjie
kaust.personMi, Jianing
kaust.personAl-Babili, Salim


Files in this item

Thumbnail
Name:
Zheng et al., 2021 JAFC Manuscript.pdf
Size:
1.833Mb
Format:
PDF
Description:
Accepted Manuscript
Embargo End Date:
2022-02-05

This item appears in the following Collection(s)

Show simple item record