Precision Phenotyping Reveals Novel Loci for Quantitative Resistance to Septoria Tritici Blotch
Type
ArticleAuthors
Yates, Steven
Mikaberidze, Alexey
Krattinger, Simon G.

Abrouk, Michael

Hund, Andreas

Yu, Kang

Studer, Bruno
Fouche, Simone
Meile, Lukas
Pereira, Danilo

Karisto, Petteri

McDonald, Bruce A.

Date
2019-09-29Submitted Date
2019-07-06Permanent link to this record
http://hdl.handle.net/10754/667227
Metadata
Show full item recordAbstract
Accurate, high-throughput phenotyping for quantitative traits is a limiting factor for progress in plant breeding. We developed an automated image analysis to measure quantitative resistance to septoria tritici blotch (STB), a globally important wheat disease, enabling identification of small chromosome intervals containing plausible candidate genes for STB resistance. 335 winter wheat cultivars were included in a replicated field experiment that experienced natural epidemic development by a highly diverse but fungicide-resistant pathogen population. More than 5.4 million automatically generated phenotypes were associated with 13,648 SNP markers to perform the GWAS. We identified 26 chromosome intervals explaining 1.9-10.6% of the variance associated with four independent resistance traits. Sixteen of the intervals overlapped with known STB resistance intervals, suggesting that our phenotyping approach can identify simultaneously (i.e., in a single experiment) many previously defined STB resistance intervals. Seventeen of the intervals were less than 5 Mbp in size and encoded only 173 genes, including many genes associated with disease resistance. Five intervals contained four or fewer genes, providing high priority targets for functional validation. Ten chromosome intervals were not previously associated with STB resistance, perhaps representing resistance to pathogen strains that had not been tested in earlier experiments. The SNP markers associated with these chromosome intervals can be used to recombine different forms of quantitative STB resistance that are likely to be more durable than pyramids of major resistance genes. Our experiment illustrates how high-throughput automated phenotyping can accelerate breeding for quantitative disease resistance.Citation
Yates, S., Mikaberidze, A., Krattinger, S. G., Abrouk, M., Hund, A., Yu, K., … McDonald, B. A. (2019). Precision Phenotyping Reveals Novel Loci for Quantitative Resistance to Septoria Tritici Blotch. Plant Phenomics, 2019, 1–11. doi:10.34133/2019/3285904Sponsors
H. Zellweger managed the wheat trial. Marion Roeder from IPK Gatersleben provided seeds and marker information for the GABI wheat panel. STB research in BAM’s lab was supported by the Swiss National Science Foundation (grants 155955, 134755, 104145, and 56874) and the ETH Zurich Research Commission (grants 12-03 and 15-02). AM and PK were supported by the Swiss National Science Foundation through Ambizione grant PZ00P3_161453.Journal
Plant PhenomicsAdditional Links
https://spj.sciencemag.org/journals/plantphenomics/2019/3285904/ae974a485f413a2113503eed53cd6c53
10.34133/2019/3285904
Scopus Count
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